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首页> 外文期刊>Cell and Tissue Research >Effect of high intensity aerobic exercise and mesterolone on remodeling of Achilles tendon of C57BL/6 transgenic mice.
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Effect of high intensity aerobic exercise and mesterolone on remodeling of Achilles tendon of C57BL/6 transgenic mice.

机译：高强度有氧运动和甲固龙对C57BL / 6转基因小鼠跟腱重构的影响。

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摘要

The effect of mesterolone and intensive treadmill training (6 weeks, 5 days/week, means: 15.82 m/min and 45.8 min/day) in Achilles tendon remodeling was evaluated. Sedentary mice treated with mesterolone (Sed-M) or vehicle (Sed-C, placebo/control) and corresponding exercised (Ex-M and Ex-C) were examined. SDS-polyacrylamide gel electrophoresis was used for determining collagen bands and hydroxyproline concentration. Collagen fibril diameter, the area and number of fibrils contained in an area probe, and the ultrastructure of fibroblasts (tenocytes) were determined. The presence of collagen was notable in the tendons of all groups. Collagen alpha(1/)alpha(2) bands in Sed-M, Ex-C, and Ex-M were higher than in Sed-C, as shown by hydroxyproline content, but collagen beta-chain appeared only in Ex-C. Noticeable bands of non-collagenous proteins were found in Sed-M and Ex-M. The number of fibrils in the area probe increased markedly in Sed-M and Ex-C (12-fold), but their diameter and area were unchanged compared with Sed-C. In Ex-M, the fibril number decreased by three-fold to 3.5-fold compared with Sed-M and Ex-C, whereas diameter and area increased. Sed-C tenocytes appeared quiescent, whereas those in the other groups seemed to be engaged in protein synthesis. The density of tenocytes was smaller in Sed-C than in Ex-C, Sed-M, and Ex-M. Thus, mechanical stimuli and mesterolone alter the morphology of tenocytes and the composition of the tendon, probably through fibrillogenesis and/or increased intermolecular cross-links. The ergogenic effect is evidenced by the activation of collagenous and non-collagenous protein synthesis and the increase in the diameter and area of collagen fibrils. This study might be relevant to clinical sports medicine.
机译：评估了甲固龙和强化跑步机训练（6周，5天/周，平均：15.82 m / min和45.8 min / day）在跟腱重塑中的效果。检查了久坐的小鼠，用美固龙（Sed-M）或媒介物（Sed-C，安慰剂/对照）治疗并进行相应的运动（Ex-M和Ex-C）。 SDS-聚丙烯酰胺凝胶电泳用于测定胶原蛋白带和羟脯氨酸浓度。测定胶原原纤维的直径，面积探针中所含的原纤维的面积和数量以及成纤维细胞（肌腱细胞）的超微结构。所有组的肌腱中均存在胶原蛋白。如羟脯氨酸含量所示，Sed-M，Ex-C和Ex-M中的胶原蛋白alpha（1 /）alpha（2）带高于Sed-C中的胶原蛋白，但胶原蛋白β链仅出现在Ex-C中。在Sed-M和Ex-M中发现了明显的非胶原蛋白条带。在Sed-M和Ex-C中，面积探针中的原纤维数量明显增加（12倍），但与Sed-C相比，它们的直径和面积没有变化。在Ex-M中，与Sed-M和Ex-C相比，原纤维数目减少了三倍至3.5倍，而直径和面积却增加了。 Sed-C肌腱细胞似乎处于静止状态，而其他组似乎参与了蛋白质合成。 Sed-C中肌腱细胞的密度小于Ex-C，Sed-M和Ex-M。因此，机械刺激和甲甾醇可能通过原纤维形成和/或分子间交联增加，改变了肌腱细胞的形态和肌腱的组成。胶原蛋白和非胶原蛋白合成的激活以及胶原蛋白原纤维直径和面积的增加证明了其产生了人为作用。这项研究可能与临床运动医学有关。

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