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首页> 外文期刊>Cell and Tissue Research >FRET analysis of interactions between actin and exon-exon-junction complex proteins in early mouse embryos.
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FRET analysis of interactions between actin and exon-exon-junction complex proteins in early mouse embryos.

机译：FRET分析早期小鼠胚胎中肌动蛋白与外显子-外显子连接复合蛋白之间的相互作用。

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Spatial interactions between nuclear actin and some proteins of the exon-exon junction complex (EJC) have been demonstrated in the nuclei of early mouse embryos by using F?rster resonance energy transfer (FRET). FRET has been registered for the pairs actin-Y14, actin-Aly/REF and actin-NXF1/TAP in nucleoplasmic, irregularly shaped zones and in nucleolus precursor bodies (NPBs). We suggest that FRET signals in the nucleoplasm correspond to the bordering zone of transcriptionally active chromatin. Detection of FRET in NPBs indicates that the list of NPB functions is broader than has been assumed to date. The FRET pattern, typical for transcriptionally active embryos, is retained after treatment with DRB (a transcription inhibitor). Spatial interactions between actin and Y14 have been found to occur in an RNA-dependent manner. Our data thus suggest that nuclear actin is directly involved in mRNA export in early mouse embryos.
机译：核肌动蛋白与外显子-外显子连接复合物（EJC）的某些蛋白质之间的空间相互作用已通过Fster共振能量转移（FRET）在早期小鼠胚胎的核中得到证实。 FRET已在核质，不规则形状区域和核仁前体（NPB）中针对肌动蛋白Y14，肌动蛋白Aly / REF和肌动蛋白NXF1 / TAP对进行了注册。我们建议核仁中的FRET信号对应于转录活性染色质的边界区域。在NPB中检测到FRET表示NPB功能列表比迄今为止假定的要广泛。在用DRB（转录抑制剂）处理后，保留了FRET模式，这对于转录活性胚胎来说是典型的。已经发现肌动蛋白和Y14之间的空间相互作用以RNA依赖性方式发生。因此，我们的数据表明核肌动蛋白直接参与早期小鼠胚胎中的mRNA输出。

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