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首页> 外文期刊>Cell and Tissue Research >Interstitial cells of Cajal mediate excitatory sympathetic neurotransmission in guinea pig prostate
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Interstitial cells of Cajal mediate excitatory sympathetic neurotransmission in guinea pig prostate

机译：Cajal间质细胞介导豚鼠前列腺兴奋性交感神经传递

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摘要

Morphological and functional studies have confirmed that interstitial cells of Cajal (ICCs) are involved in many enteric motor neurotransmission pathways. Recent investigations have demonstrated that human and guinea pig prostate glands possess a distinct cell type with morphological and immunological similarities to ICCs. These prostate ICCs have a close relationship with nerve bundles and smooth muscle cells. Prostate smooth muscle tone is largely induced by stimulation from the sympathetic nervous system, which releases excitatory norepinephrine (NE) to act on the α1-adrenoceptor. We have performed morphological and functional experiments to determine the role of ICCs in sympathetic neurotransmission in the guinea pig prostate based on the hypothesis that prostate ICCs act as mediators of sympathetic neurotransmission. Immunohistochemistry revealed many close points of contact between ICCs and sympathetic nerve bundles and smooth muscle cells. Double-labeled sections revealed that α1-adrenoceptor and the gap junction protein connexin 43 were expressed in prostate ICCs. Surprisingly, prostate ICCs co-expressed tyrosine hydroxylase and dopamine β-hydroxylase, two markers of sympathetic neurons. Functionally, the application of NE evoked a large single inward current in isolated prostate ICCs in a dose-dependent manner. The inward current evoked by NE was mediated via the activation of α1-adrenoceptors, because it was abolished by the non-specific α-adrenoceptor antagonist, phentolamine and the specific α1-adrenoceptor antagonist, prazosin. Thus, ICCs in the guinea pig prostate are target cells for prostate sympathetic nerves and possess the morphological and functional characteristics required to mediate sympathetic signals.
机译：形态学和功能研究已证实，Cajal间质细胞（ICC）参与了许多肠运动神经传递途径。最近的研究表明，人和豚鼠前列腺具有与ICC形态和免疫学相似的独特细胞类型。这些前列腺ICC与神经束和平滑肌细胞密切相关。交感神经系统的刺激在很大程度上诱发了前列腺的平滑肌张力，它释放了兴奋性去甲肾上腺素（NE）以作用于α1-肾上腺素受体。基于前列腺ICC充当交感神经传递介体的假设，我们已经进行了形态学和功能实验，以确定ICC在豚鼠前列腺交感神经传递中的作用。免疫组织化学显示，ICC与交感神经束和平滑肌细胞之间有许多紧密的接触点。双标记切片显示，α1肾上腺素受体和间隙连接蛋白连接蛋白43在前列腺ICC中表达。令人惊讶的是，前列腺ICCs共表达了交感神经元的两个标志物酪氨酸羟化酶和多巴胺β-羟化酶。在功能上，NE的应用以剂量依赖的方式在孤立的前列腺ICC中引起大的单向内流。 NE诱发的内向电流是通过α1-肾上腺素受体的激活介导的，因为它被非特异性α-肾上腺素受体拮抗剂酚妥拉明和特异性α1-肾上腺素受体拮抗剂哌唑嗪所消除。因此，豚鼠前列腺中的ICC是前列腺交感神经的靶细胞，并具有介导交感信号所需的形态和功能特征。

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