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首页> 外文期刊>Cell and Tissue Research >Regulation of LH/FSH expression by secretoglobin 3A2 in the mouse pituitary gland
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Regulation of LH/FSH expression by secretoglobin 3A2 in the mouse pituitary gland

机译：分泌蛋白3A2在小鼠垂体中调节LH / FSH表达

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Secretoglobin (SCGB) 3A2 was originally identified as a downstream target for the homeodomain transcription factor NKX2-1 in the lung. NKX2-1 plays a role in the genesis and expression of genes in the thyroid, lung and ventral forebrain; Nkx2-1-null mice have no thyroid and pituitary and severely hypoplastic lungs and hypothalamus. To demonstrate whether SCGB3A2 plays any role in pituitary hormone production, NKX2-1 and SCGB3A2 expression in the mouse pituitary gland was examined by immunohistochemical analysis and RT-PCR. NKX2-1 was localized in the posterior pituitary lobe, whereas SCGB3A2 was observed in both anterior and posterior lobes as shown by immunohistochemistry and RT-PCR. Expression of CCAAT-enhancer binding proteins (C/EBPs), which regulate mouse Scgb3a2 transcription, was also examined by RT-PCR. C/EBPβ, γ, δ and ζ were expressed in the adult mouse pituitary gland. SCGB3A2 was expressed in the anterior and posterior lobes from postnatal days 1 and 5, respectively and the areas where SCGB3A2 expression was found coincided with the area where FSH-secreting cells were found. Double-staining for SCGB3A2 and pituitary hormones revealed that SCGB3A2 was mainly localized in gonadotrophs in 49 % of FSH-secreting cells and 47 % of LH-secreting cells. In addition, SCGB3A2 dramatically inhibited LH and FSH mRNA expression in rat pituitary primary cell cultures. These results suggest that SCGB3A2 regulates FSH/LH production in the anterior pituitary lobe and that transcription factors other than NKX2-1 may regulate SCGB3A2 expression.
机译：分泌血红蛋白（SCGB）3A2最初被确定为肺中同源域转录因子NKX2-1的下游靶标。 NKX2-1在甲状腺，肺和腹前脑的基因的产生和表达中起作用。 Nkx2-1无效的小鼠没有甲状腺和垂体，肺和下丘脑严重发育不良。为了证明SCGB3A2是否在垂体激素产生中发挥任何作用，通过免疫组织化学分析和RT-PCR检测了小鼠垂体中NKX2-1和SCGB3A2的表达。 NKX2-1位于垂体后叶，而免疫组化和RT-PCR显示在前叶和后叶均观察到SCGB3A2。还通过RT-PCR检查了调节小鼠Scgb3a2转录的CCAAT增强子结合蛋白（C / EBP）的表达。 C /EBPβ，γ，δ和ζ在成年小鼠垂体中表达。从出生后的第1天和第5天开始，SCGB3A2在前叶和后叶中表达，发现SCGB3A2表达的区域与发现FSH分泌细胞的区域一致。对SCGB3A2和垂体激素的双重染色显示，SCGB3A2主要位于49％的FSH分泌细胞和47％的LH分泌细胞的性腺营养体中。此外，SCGB3A2在大鼠垂体原代细胞培养物中显着抑制LH和FSH mRNA表达。这些结果表明，SCGB3A2调节垂体前叶中FSH / LH的产生，除NKX2-1以外的转录因子也可能调节SCGB3A2的表达。

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