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首页> 外文期刊>Cell and Tissue Research >Intramyocardial injection of hypoxia-preconditioned adipose-derived stromal cells treats acute myocardial infarction: an in vivo study in swine.
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Intramyocardial injection of hypoxia-preconditioned adipose-derived stromal cells treats acute myocardial infarction: an in vivo study in swine.

机译：心肌内注射缺氧预处理的脂肪来源的基质细胞可治疗急性心肌梗塞：一项关于猪的体内研究。

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摘要

Hypoxic preconditioning is a promising method for improving the anti-apoptotic and paracrine signaling capabilities of adipose-derived stromal cells (ADSCs). The purpose of this study was to analyze the influence of different hypoxic conditions on ADSCs and the therapeutic effects of hypoxia-preconditioned ADSCs (HPADSCs) on an animal model of myocardial infarction (MI). For the in vitro studies, ADSCs were divided into five groups and cultured in different oxygen concentrations (1, 3, 5, 10, and 21 %). After 24 h, RT-PCR and western blots showed that 3 % oxygen preconditioning could improve the viability and cytokine secretion of the ADSCs. A Matrigel assay indicated that the HPADSC-conditioned medium could stimulate endothelial cells to form capillary-like tubes. For the in vivo studies, MI was induced by coronary occlusion in 24 mature Chinese minipigs. The animals were divided into three groups and treated by intramyocardial injection with vehicle alone (saline group), with 1 × 10(8) ADSCs cultured in normoxic conditions (ADSCs group) or with 1 × 10(8) ADSCs precultured in 3 % oxygen (HPADSCs group). SPECT and echocardiography demonstrated that cardiac function was improved significantly in the HPADSC transplant group compared with the vehicle control group (P < 0.05). Immunofluorescence showed fewer apoptotic cells and more small- to medium-sized vessels in the HPADSC transplantation group (P < 0.05). Three percent oxygen is the optimum preconditioning treatment for ADSCs. HPADSC transplantation can prevent ventricular remodeling and reduce the infarct size.
机译：缺氧预处理是改善脂肪来源的基质细胞（ADSC）的抗凋亡和旁分泌信号传导能力的一种有前途的方法。这项研究的目的是分析不同的低氧条件对ADSCs的影响以及缺氧预处理的ADSCs（HPADSCs）对心肌梗塞（MI）动物模型的治疗效果。对于体外研究，将ADSC分为五组，并以不同的氧气浓度（1、3、5、10和21％）进行培养。 24小时后，RT-PCR和蛋白质印迹显示3％的氧气预处理可以改善ADSC的活力和细胞因子分泌。基质胶测定表明，HPADSC条件培养基可以刺激内皮细胞形成毛细管样管。在体内研究中，MI是由24只成熟的中国小型猪的冠状动脉闭塞引起的。将动物分为三组，并通过心肌内注射单独用赋形剂治疗（盐水组），在常氧条件下培养1×10（8）ADSC（ADSCs组），或在3％氧气中预培养1×10（8）ADSCs （HPADSCs组）。 SPECT和超声心动图显示，HPADSC移植组的心脏功能明显优于媒介物对照组（P <0.05）。免疫荧光显示，HPADSC移植组的凋亡细胞更少，而中小血管则更多（P <0.05）。百分之三的氧气是ADSC的最佳预处理方法。 HPADSC移植可以防止心室重构，并缩小梗塞面积。

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