In vivo and in vitro radioprotective effects of the prostaglandin E1 analogue misoprostol in DNA repair-proficient and -deficient rodent cell systems.

van Buul PP; Sankaranarayanan K; van Duyn-Goedhart A

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In vivo and in vitro radioprotective effects of the prostaglandin E1 analogue misoprostol in DNA repair-proficient and -deficient rodent cell systems.

机译：前列腺素E1类似物米索前列醇在DNA修复有效和缺陷的啮齿动物细胞系统中的体内和体外放射防护作用。

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The radioprotective effect of a stable prostaglandin E(1) analogue, misoprostol, was studied in cells from mice with severe combined immunodeficiency (SCID) and in normal cells using X-ray-induced chromosomal aberrations and/or cell killing as the end points. The results clearly show misoprostol-induced radioprotective effects in spermatocytes of the first meiotic division when analyzed for X-ray-induced chromosomal aberrations. The protective effect was independent of Trp53 (formerly known as p53) status. Since spermatocytes are relatively easy to isolate, this appears to be a suitable in vivo model that will allow biochemical studies of the mechanisms involved in radioprotection mediated by misoprostol. Using transfected CHO-K1 cells that stably express a PGE(2) receptor (CPE cells), significant radioprotection mediated by misoprostol from both chromosome breakage and cell death could be demonstrated under in vitro conditions. In addition, evidence was obtained indicating that the degree of radioprotection was dependent on the cell cycle and that S-phase cells were less responsive to misoprostol-mediated radioprotection. These results suggest that CPE cells may be a suitable in vitro model for further studies on the cellular pathways involved in radioprotection by misoprostol in particular and prostaglandins in general.

机译：使用X射线诱导的染色体畸变和/或细胞杀伤作为终点，研究了具有严重联合免疫缺陷（SCID）的小鼠细胞和正常细胞中稳定的前列腺素E（1）类似物米索前列醇的放射防护作用。结果清楚地表明，当分析X射线诱导的染色体畸变时，米索前列醇在第一减数分裂分裂的精细胞中诱导的放射防护作用。保护作用与Trp53（以前称为p53）状态无关。由于精母细胞相对容易分离，因此这似乎是合适的体内模型，它将允许对米索前列醇介导的放射防护机制进行生化研究。使用稳定表达PGE（2）受体（CPE细胞）的转染CHO-K1细胞，在体外条件下可以证明米索前列醇从染色体断裂和细胞死亡中介导的显着放射防护。另外，获得的证据表明放射防护的程度取决于细胞周期，并且S期细胞对米索前列醇介导的放射防护反应较弱。这些结果表明，CPE细胞可能是合适的体外模型，用于进一步研究与米索前列醇特别是一般前列腺素的放射防护有关的细胞途径。

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《Radiation Research: Official Organ of the Radiation Research Society》 |1999年第4期|共6页
作者
van Buul PP; Sankaranarayanan K; van Duyn-Goedhart A;
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正文语种 eng
中图分类辐射与测量;
关键词
Animal; Cell Cycle: Radiation effects; Chromosome Aberrations; DNA Repair; Male; Mice; Mice; Inbred C57BL; Mice; Knockout; Mice; SCID; Misoprostol: Pharmacology; Protein p53: Metabolism; Radiation-Protective Agents: Pharmacology;

机译：动物;细胞周期：辐射效应;染色体畸变;DNA修复;雄性;小鼠;小鼠;近交C57BL;小鼠;敲除;小鼠;SCID;米索前列醇：药理学;蛋白质p53：新陈代谢;辐射防护剂：药理学;

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1. In vivo and in vitro radioprotective effects of the prostaglandin E1 analogue misoprostol in DNA repair-proficient and -deficient rodent cell systems. [J] . van Buul PP, Sankaranarayanan K, van Duyn-Goedhart A Radiation Research: Official Organ of the Radiation Research Society . 1999,第4期

机译：前列腺素E1类似物米索前列醇在DNA修复有效和缺陷的啮齿动物细胞系统中的体内和体外放射防护作用。
2. Attenuation of ischemic liver injury by prostaglandin E1 analogue, misoprostol, and prostaglandin I2 analogue, OP-41483. [J] . Totsuka E, Todo S, Zhu Y, Journal of the American College of Surgeons . 1998,第3期

机译：前列腺素E1类似物，米索前列醇和前列腺素I2类似物OP-41483可减轻缺血性肝损伤。
3. The role of adenosine 3′:5′-cyclic monophosphate in the division of WI 38 cells. The cellular response to prostaglandin E1 and the effects of an cyclic adenosine 3′:5′-cyclic monophosphate analogue and prostaglandin E1 on cell division [J] . Alexander M. Rutenburg, Linda Taylor, Mary J. Kurtz, The biochemical journal . 1974,第2期

机译：腺苷3'：5'-环一磷酸在WI 38细胞分裂中的作用。细胞对前列腺素E1的反应以及环状腺苷3'：5'-环一磷酸类似物和前列腺素E1对细胞分裂的影响
4. In vitro and in vivo evaluation of microspheric DNA vaccine delivery systems. [C] . K.N.Atuah, C.J.McHugh, E.Walter, International symposium on controlled release of bioactive materials . 2000

机译：体外和体内微球DNA疫苗递送系统的体内评价。
5. Characterization of the interaction of the chemotherapeutic drug mitomycin C with DNA in vitro and in vivo and effects on specific DNA-protein interactions [D] . Warren, Amy J. 1996

机译：体外和体内化学治疗药物丝裂霉素C与DNA相互作用的特性及其对特定DNA-蛋白质相互作用的影响
6. The prostaglandin E1 analogue misoprostol regulates inflammatory cytokines and immune functions in vitro like the natural prostaglandins E1 E2 and E3. [O] . D R Haynes, M W Whitehouse, B Vernon-Roberts 1992

机译：前列腺素E1类似物米索前列醇像天然前列腺素E1E2和E3一样在体外调节炎症细胞因子和免疫功能。
7. Attenuation of ischemic liver injury by prostaglandin E1 analogue, misoprostol, and prostaglandin I2 analogue, OP-41483 [O] . Totsuka E, Todo S, Zhu Y, 1998

机译：前列腺素E1类似物，米索前列醇和前列腺素I2类似物OP-41483对缺血性肝损伤的缓解作用
8. In vivo and in vitro effects of staurosporine on the DNA distributions in and proliferation of various rodent cell populations [R] . Kim, J. M., Archuleta, D., Lehnert, N. M., 1994

机译：星形孢菌素对各种啮齿动物细胞群的DNa分布和增殖的体内和体外作用

In vivo and in vitro radioprotective effects of the prostaglandin E1 analogue misoprostol in DNA repair-proficient and -deficient rodent cell systems.

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