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首页> 外文期刊>Cellular immunology >New approach to study of T cellular immunity development: parallel investigation of lymphoid organ formation and changes in immune proteasome amount in rat early ontogenesis.
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New approach to study of T cellular immunity development: parallel investigation of lymphoid organ formation and changes in immune proteasome amount in rat early ontogenesis.

机译:研究T细胞免疫发展的新方法:大鼠早期肿瘤发生中淋巴器官形成和免疫蛋白酶体量变化的平行研究。

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摘要

The expression pattern and distribution of proteasome immune subunits LMP7 and LMP2 in the developing rat spleen and liver as well as the periarterial lymphoid sheath formation were investigated. LMP7 and LMP2 were detected by immunoblotting in the spleen on the 21st embryonic day and during the first postnatal days in equal amounts. Their levels increased by the 8th and 18th postnatal days. Double immunofluorescent labeling the spleen cells revealed LMP7 and LMP2 in T and B lymphocytes localized in the red pulp in embryogenesis. Few T lymphocytes were discovered in periarterial zones on the 8th postnatal day. T lymphocytes filled these zones and formed lymphoid sheaths by the 18-19th day. In the liver, LMP7 and LMP2 were revealed by the 17-19th postnatal day. Immunofluorescent analysis showed their presence in hepatocytes at this period. The data suggest that T cell-mediated immune response in relation to hepatocytes is possible beginning from 18th to 19th postnatal day.
机译:研究了在发育中的大鼠脾脏和肝脏中蛋白酶体免疫亚基LMP7和LMP2的表达模式和分布,以及动脉周围淋巴样鞘的形成。 LMP7和LMP2在第21个胚胎日和出生后的头几天通过免疫印迹检测到的脾脏中的含量相等。出生后第8天和第18天,它们的水平增加。脾细胞的双重免疫荧光标记显示了胚胎发生中位于红髓中的T和B淋巴细胞中的LMP7和LMP2。出生后第8天在动脉周围区域发现了很少的T淋巴细胞。在第18-19天,T淋巴细胞充满了这些区域并形成了淋巴样鞘。在肝脏中,LMP7和LMP2在出生后的第17-19天显示出来。免疫荧光分析表明在此期间它们存在于肝细胞中。数据表明,从出生后第18天到第19天开始,与肝细胞相关的T细胞介导的免疫反应是可能的。

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