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首页> 外文期刊>Cell cycle >PGRMC1 participates in late events of bovine granulosa cells mitosis and oocyte meiosis
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PGRMC1 participates in late events of bovine granulosa cells mitosis and oocyte meiosis

机译：PGRMC1参与牛颗粒细胞有丝分裂和卵母细胞减数分裂的晚期事件

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Progesterone Receptor Membrane Component 1 (PGRMC1) is expressed in both oocyte and ovarian somatic cells, where it is found in multiple cellular sub-compartments including the mitotic spindle apparatus. PGRMC1 localization in the maturing bovine oocytes mirrors its localization in mitotic cells, suggesting a possible common action in mitosis and meiosis. To test the hypothesis that altering PGRMC1 activity leads to similar defects in mitosis and meiosis, PGRMC1 function was perturbed in cultured bovine granulosa cells (bGC) and maturing oocytes and the effect on mitotic and meiotic progression assessed. RNA interference-mediated PGRMC1 silencing in bGC significantly reduced cell proliferation, with a concomitant increase in the percentage of cells arrested at G2/M phase, which is consistent with an arrested or prolonged M-phase. This observation was confirmed by time-lapse imaging that revealed defects in late karyokinesis. In agreement with a role during late mitotic events, a direct interaction between PGRMC1 and Aurora Kinase B (AURKB) was observed in the central spindle at of dividing cells. Similarly, treatment with the PGRMC1 inhibitor AG205 or PGRMC1 silencing in the oocyte impaired completion of meiosis I. Specifically the ability of the oocyte to extrude the first polar body was significantly impaired while meiotic figures aberration and chromatin scattering within the ooplasm increased. Finally, analysis of PGRMC1 and AURKB localization in AG205-treated oocytes confirmed an altered localization of both proteins when meiotic errors occur. The present findings demonstrate that PGRMC1 participates in late events of both mammalian mitosis and oocyte meiosis, consistent with PGRMC1's localization at the mid-zone and mid-body of the mitotic and meiotic spindle.
机译：孕激素受体膜成分1（PGRMC1）在卵母细胞和卵巢体细胞中都有表达，在包括有丝分裂纺锤体在内的多个细胞子隔室中都可以找到。 PGRMC1在成熟的牛卵母细胞中的定位反映了其在有丝分裂细胞中的定位，表明在有丝分裂和减数分裂中可能有共同作用。为了检验这一假设，即改变PGRMC1活性会导致类似的有丝分裂和减数分裂缺陷，在培养的牛颗粒细胞（bGC）和成熟的卵母细胞中扰动了PGRMC1功能，并评估了对有丝分裂和减数分裂进程的影响。 bGC中RNA干扰介导的PGRMC1沉默显着降低了细胞增殖，并伴随着G2 / M期停滞的细胞百分比增加，这与M期停滞或延长相一致。延时成像证实了这一发现，该成像揭示了晚期核运动的缺陷。与在晚期有丝分裂事件中的作用一致，在分离细胞的中心纺锤体中观察到了PGRMC1和极光激酶B（AURKB）之间的直接相互作用。类似地，在卵母细胞中用PGRMC1抑制剂AG205或PGRMC1沉默处理会减缓减数分裂I的完成。特别是，卵母细胞挤出第一个极体的能力会显着受损，而减数分裂的像差和卵质中的染色质散射会增加。最后，对AG205处理的卵母细胞中PGRMC1和AURKB定位的分析证实，当减数分裂错误发生时，两种蛋白质的定位都发生了改变。本研究结果表明，PGRMC1参与哺乳动物有丝分裂和卵母细胞减数分裂的晚期事件，这与PGRMC1在有丝分裂和减数分裂纺锤体的中部和中部的定位一致。

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