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首页> 外文期刊>Cellular immunology >Activation of myeloid dendritic cells by deoxynucleic acids from Cordyceps sinensis via a Toll-like receptor 9-dependent pathway.
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Activation of myeloid dendritic cells by deoxynucleic acids from Cordyceps sinensis via a Toll-like receptor 9-dependent pathway.

机译：冬虫夏草的脱氧核酸通过Toll样受体9依赖性途径激活髓样树突状细胞。

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摘要

The mechanism by which host cells recognize Cordyceps sinensis, a Chinese herbal medicine that is known to exhibit immunomodulating activity, remains poorly understood. In this study, we investigated whether the DNA of this fungus could activate mouse bone marrow-derived dendritic cells (BM-DCs). Upon stimulation with C. sinensis DNA, BM-DCs released IL-12p40 and TNF-alpha and expressed CD40. Cytokine production and CD40 expression were attenuated by chloroquin and bafilomycin A. Activation of BM-DCs by C. sinensis DNA was almost completely abrogated in TLR9KO mice. According to a luciferase reporter assay, C. sinensis DNA activated NF-kappaB in HEK293T cells transfected with the TLR9 gene. Finally, a confocal microscopic analysis showed that C. sinensis DNA was co-localized with CpG-ODN and partly with TLR9 and LAMP-1, a late endosomal marker, in BM-DCs. Our results demonstrated that C. sinensis DNA caused activation of BM-DCs in a TLR9-dependent manner.
机译：宿主细胞识别冬虫夏草的机理尚不清楚，冬虫夏草是一种已知具有免疫调节活性的中草药。在这项研究中，我们调查了这种真菌的DNA是否可以激活小鼠骨髓来源的树突状细胞（BM-DCs）。在用中华梭菌DNA刺激后，BM-DCs释放IL-12p40和TNF-α并表达CD40。细胞因子的产生和CD40的表达被氯喹和巴氟霉素A所减弱。中华绒螯蟹DNA对BM-DCs的激活在TLR9KO小鼠中几乎被完全废除。根据荧光素酶报告基因测定，中华绒螯蟹DNA激活了用TLR9基因转染的HEK293T细胞中的NF-κB。最后，共聚焦显微镜分析表明，中华绒螯蟹DNA与CpG-ODN共定位，部分与TLR9和晚期内体标记物LAMP-1共定位于BM-DC中。我们的结果表明中华绒螯蟹DNA以TLR9依赖性方式引起BM-DCs的激活。

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