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首页> 外文期刊>Radiation Research: Official Organ of the Radiation Research Society >MOLECULAR ANALYSIS OF GAMMA-RAY-INDUCED MUTATIONS AT THE HPRT LOCUS IN PRIMARY HUMAN SKIN FIBROBLASTS BY MULTIPLEX POLYMERASE CHAIN REACTION
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MOLECULAR ANALYSIS OF GAMMA-RAY-INDUCED MUTATIONS AT THE HPRT LOCUS IN PRIMARY HUMAN SKIN FIBROBLASTS BY MULTIPLEX POLYMERASE CHAIN REACTION

机译：多重聚合酶链反应在人类皮肤成纤维细胞中HPRT部位γ射线诱变的分子分析

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A total of 153 hprt mutants (23 spontaneous, 130 gamma-ray-induced) of primary human skin fibroblasts were isolated and genetic alterations at the locus were studied by multiplex polymerase chain reaction (PCR). The analyses showed that 51% (66/130) of gamma-ray-induced genetic changes were large deletions, whereas the majority of spontaneous mutants (21/23) exhibited point mutations. The spectrum of large genetic alterations appeared to be dependent on dose in gamma-ray-induced (1-4 Gy) mutations; mutants with complete loss of the hprt locus comprised 21 (3/14) or 39% (15/38) of clones isolated after irradiation with 1 or 4 Gy, respectively. The frequency of partial deletions was found to be higher in the mutants isolated from clones irradiated with 2 Gy (38%) than from those irradiated with 4 Gy (8%). Mapping of all intragenic deletion breakpoints exhibited a nonrandom distribution of breakpoints toward the 3' end of the hprt gene. [References: 46]
机译：分离出总共153个hprt突变体（23个自发，130个伽马射线诱导）的原代人皮肤成纤维细胞，并通过多重聚合酶链反应（PCR）研究了该位点的遗传改变。分析表明，有51％（66/130）的伽马射线引起的遗传变化是大缺失，而大多数自发突变体（21/23）表现出点突变。大的遗传改变的谱似乎取决于伽马射线诱导的（1-4 Gy）突变的剂量。 hprt基因座完全丢失的突变体分别包含21（3/14）或39％（15/38）的克隆，分别用1或4 Gy照射后分离出克隆。发现从用2 Gy辐照的克隆（38％）分离的突变体中，比从用4 Gy辐照的克隆（8％）分离的突变体，其部分缺失的频率更高。所有基因内缺失断点的定位均显示出断点朝着hprt基因3'端的非随机分布。 [参考：46]

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