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首页> 外文期刊>Cell and Tissue Research >Retinoic acid as a survival factor in neuronal development of the grasshopper, Locusta migratoria.
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Retinoic acid as a survival factor in neuronal development of the grasshopper, Locusta migratoria.

机译:维甲酸作为蝗虫神经元发育的存活因子。

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摘要

Based on experience with cell cultures of adult insect neurons, we develop a serum-free culture system for embryonic locust neurons. Influences of trophic substances on survival and neurite outgrowth of developing neurons are investigated. For the first time, a positive trophic effect of 9-cis retinoic acid (9-cis RA) was shown in vitro on embryonic neurons of an insect. We observed longer cell survival of 50 % developmental stage neurons in cultures supplemented with 0.3 nM 9-cis RA. Furthermore, an influence on neuron morphology was revealed, as the addition of 9-cis RA to cell culture medium led to an increase in the number of neurites per cell. Although an RA receptor gene, LmRXR (Locusta migratoria retinoid X receptor), was expressed in the central nervous system throughout development, the influence of 9-cis RA on neuronal survival and outgrowth was restricted to 50 % stage embryonic cells.
机译:基于对成虫昆虫神经元细胞培养的经验,我们为胚胎蝗神经元开发了无血清培养系统。研究了营养物质对发育中的神经元存活和神经突生长的影响。首次在体外对昆虫的胚胎神经元显示了9-顺式视黄酸(9-顺式RA)的正营养作用。我们观察到在补充0.3 nM 9-顺式RA的培养物中50%发育阶段神经元的细胞存活时间更长。此外,揭示了对神经元形态的影响,因为向细胞培养基中添加9-顺式RA导致每个细胞的神经突数量增加。尽管RA受体基因LmRXR(Locusta migratoria维甲酸X受体)在整个发育过程中都在中枢神经系统中表达,但9-顺式RA对神经元存活和生长的影响仅限于50%阶段的胚胎细胞。

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