首页> 外文期刊>Range Management & Agroforestry >Molecular and embryological analyses of rare sexual plant in Buffelgrass (Cenchrus ciliaris L.).
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Molecular and embryological analyses of rare sexual plant in Buffelgrass (Cenchrus ciliaris L.).

机译:Buffelgrass( Cenchrus ciliaris L.)中稀有有性植物的分子和胚胎学分析。

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Buffelgrass (Cenchrus ciliaris L.) is an important forage grass, which reproduces predominantly through apomixis. Apomixis in C. ciliaris limits possibilities of genetic improvement of this species by hybridization and studying phylogenetic relationship. Obligate apomictic and sexual plants are required for the development of mapping population for genetic studies in apomixis. We identified an obligate sexual plant of C. ciliaris using pistil-clearing technique and DNA profile of this rare sexual plant was developed using RAPD technique. A 10-mer primer OPJ-13 produced a plant-specific band of about 0.22 kb, while OPP-14 primer produced specific band of about 1.2 kb. The DNA profile can be used for identification of this plant and may be useful in the genetic studies involving this plant as a parent.
机译:水牛草(Cuffrus ciliaris L.)是一种重要的牧草,主要通过无融合生殖繁殖。 C中无融合生殖。纤毛通过杂交和研究系统发生关系限制了该物种遗传改良的可能性。发展无融合生殖基因研究的作图种群的发展需要专性无融合生殖和有性植物。我们鉴定出一种专性的iC植物。使用雌蕊清除技术的纤毛,并使用RAPD技术开发了这种稀有性植物的DNA谱。 10聚体引物OPJ-13产生约0.22kb的植物特异性条带,而OPP-14引物产生约1.2kb的特异性条带。 DNA概况可用于鉴定该植物,并且可用于涉及该植物作为亲本的遗传研究中。

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