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首页> 外文期刊>Livestock Science >Effect of dietary forage-to-concentrate ratios on urinary excretion of purine derivatives and microbial nitrogen yields in the rumen of Dorper crossbred sheep.
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Effect of dietary forage-to-concentrate ratios on urinary excretion of purine derivatives and microbial nitrogen yields in the rumen of Dorper crossbred sheep.

机译：日粮饲草浓度比对多珀杂交绵羊瘤胃中嘌呤衍生物尿排泄和微生物氮产量的影响。

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This study investigated the effects of various dietary forage-to-concentrate ratios (F:C) on urinary excretion of purine derivatives (PD) and microbial nitrogen (MN) yields from the rumen of Dorper x thin-tailed Han crossbred sheep. Twelve Dorper x thin-tailed Han crossbred ram lambs (47.2+or-1.0 kg) fitted with ruminal and duodenal cannulae were randomly assigned to 12 levels of dietary F:C in an incomplete Latin-square experimental design (12 lambs x 4 periods). Digestibility trials were conducted and MN was estimated using either 15N or PD as markers. Urinary excretion of allantoin and total PD increased (P<0.05) with decreasing F:C. Urinary excretion of uric acid or xanthine plus hypoxanthine was unaffected by F:C (P>0.05). MN yields estimated using 15N as a marker were greater than those predicted from urinary PD (12.5 vs. 11.5 g/d, P<0.05), but the former was more variable than the latter (S.E.=0.66 vs. 0.45, respectively). A linear correlation existed between MN estimated by 15N and urinary excretion of PD: MN (g/d)=-0.521+1.493 x PD (mmol/d) (r2=0.86, P<0.05). The purine nitrogen index (PNI: PD nitrogen/urinary N) was linearly correlated with nitrogen capture efficiency calculated from either 15N or PD (r2=0.60 and 0.77, respectively). Results suggest that urinary PD is an accurate indicator of MN from the rumen of Dorper crossbred sheep, and PNI reflected the conversion of nitrogen degradation to MN in the rumen.
机译：这项研究调查了各种饲料草料-浓度比（F：C）对Dorper x稀尾汉杂种绵羊瘤胃中嘌呤衍生物（PD）和微生物氮（MN）产量尿排泄的影响。在不完全的拉丁方实验设计中，将十二只Dorper x细尾汉杂交羔羊（47.2+或-1.0千克）和瘤胃和十二指肠插管随机分配到12种膳食F：C水平（12只羔羊x 4期） 。进行了消化性试验，并使用 15 N或PD作为标记物估算了MN。尿尿排泄量和总PD随F：C降低而增加（P <0.05）。尿酸或黄嘌呤加次黄嘌呤的尿排泄不受F：C的影响（P> 0.05）。以 15 N为标记物估算的MN产量要高于尿PD预测的产量（12.5 vs. 11.5 g / d，P <0.05），但前者的可变性要比后者高（SE =分别为0.66和0.45）。 15 N估计的MN与PD的尿排泄之间存在线性关系：MN（g / d）=-0.521 + 1.493 x PD（mmol / d）（r 2 = 0.86，P <0.05）。嘌呤氮指数（PNI：PD氮/尿N）与 15 N或PD计算的氮捕获效率线性相关（r 2 = 0.60和0.77 ）。结果表明，尿PD是Dorper杂交绵羊瘤胃中MN的准确指标，而PNI反映了瘤胃中氮降解向MN的转化。

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