...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell cycle >The yeast kinase Swe1 is required for proper entry into cell cycle after arrest due to ribosome biogenesis and protein synthesis defects.
【24h】

The yeast kinase Swe1 is required for proper entry into cell cycle after arrest due to ribosome biogenesis and protein synthesis defects.

机译：酵母激酶Swe1是由于核糖体生物发生和蛋白质合成缺陷而在逮捕后正确进入细胞周期所必需的。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Sda1 is an essential protein required for cell cycle progression in Saccharomyces cerevisiae. Here, we show that the sda1-1 mutation causes a defect in the formation and nuclear export of 60S ribosomal subunits. Moreover, the sda1-1, but also other mutants defective in ribosome biogenesis (e.g., rix1-1 and tif6Delta), exhibit a G1 arrest, which could be the consequence of impaired ribosome biogenesis. Interestingly, additional deletion of the non-essential Swe1 kinase, the homolog of S. pombe Wee1, causes a pronounced delay in entering a new cell cycle in sda1-1, rix1-1 and tif6Delta cells, when shifted back from restrictive to permissive conditions. However, such a prolonged delay is independent of the Tyr19 phosphorylation in Cdc28. Moreover, the lack of Swe1 causes delay in budding and DNA replication in cells released from the G1 arrest due to the block of protein synthesis. Our data suggest that Swe1 is required for timely entry into cell cycle after a G1 arrest caused by impairment in pre-60S biogenesis and in protein synthesis. Therefore we propose that Swe1, which is required for coordination of cell growth and cell division in G2/M, also has a role in the beginning of the cell cycle.
机译：Sda1是酿酒酵母细胞周期进程所需的必需蛋白质。在这里，我们显示sda1-1突变导致60S核糖体亚基的形成和核输出中的缺陷。此外，sda1-1和其他在核糖体生物发生中有缺陷的突变体（例如rix1-1和tif6Delta）表现出G1阻滞，这可能是核糖体生物发生受损的结果。有趣的是，非必需的Swe1激酶（即粟酒裂殖酵母Wee1的同系物）的额外缺失会导致从sda1-1，rix1-1和tif6Delta细胞进入新的细胞周期时出现明显的延迟，当从限制性条件转变为宽松条件时。但是，这种延长的延迟与Cdc28中的Tyr19磷酸化无关。此外，由于蛋白质合成的阻滞，缺乏Swe1会导致从G1阻滞释放的细胞中芽和DNA复制的延迟。我们的数据表明，Swe1是在60S之前的生物合成和蛋白质合成受损导致的G1停滞后及时进入细胞周期所必需的。因此，我们提出，Swe1是协调细胞生长和G2 / M中细胞分裂所必需的，它在细胞周期的开始也具有作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号