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首页> 外文期刊>Livestock Science >Effect of sex steroids on expression of sulfotransferase 2B1 immunoreactive protein in primary cultured porcine hepatocytes
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Effect of sex steroids on expression of sulfotransferase 2B1 immunoreactive protein in primary cultured porcine hepatocytes

机译：性激素对原代培养猪肝细胞中磺基转移酶2B1免疫反应蛋白表达的影响

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摘要

An excessive accumulation of androstenone (5l-androst-16-en-3-one) in pig adipose tissue is one of the two major contributors to the phenomenon of boar taint. High levels of adipose tissue androstenone have been related to a low rate of hepatic androstenone metabolism, which includes two stages: oxidative and conjugative. Sulfotransferases (SULTs), alongside with other specific enzymes, play the key role in the conjugative stage of androstenone metabolism. The present study investigated the mechanism regulating expression of sulfotransferase 2B1 (SULT2B1) immunoreactive protein using primary cultured pig hepatocytes as a model system. A specific objective was to determine whether the expression of pig hepatic SULT2B1 is regulated by the sex steroids; androstenone, testosterone and estrone sulphate. The study was performed on entire male pigs of a Large White (40%)xLandrace (40%)xDuroc (20%) cross-breed, average carcass weight 72.2 kg. The study shows that SULT2B1 immunoreactive protein expression can be induced by testosterone (final concentrations, 10 and 500 nM) and repressed by estrone sulphate (final concentration, 100 nM). Androstenone had no significant effect on SULT2B1 immunoreactive protein expression in the range of concentration, 10 nM to 1 oM. Time-courses (0 to 48 h) of steroid effects were investigated. The maximum effects of testosterone and estrone sulphate were observed in 24 h after the steroid treatments. This study provides direct evidence for involvement of sex steroids in the regulation of porcine hepatic SULTs.
机译：猪脂肪组织中雄烯酮（5l-androst-16-en-3-one）的过度积累是造成公猪异味现象的两个主要因素之一。脂肪组织中雄烯酮的高含量与肝脏雄烯酮的低代谢有关，这包括两个阶段：氧化阶段和结合阶段。磺基转移酶（SULTs）与其他特定酶一起，在雄烯酮酮代谢的共轭阶段中起关键作用。本研究使用原代培养的猪肝细胞作为模型系统研究了调节磺基转移酶2B1（SULT2B1）免疫反应蛋白表达的机制。一个特定的目标是确定猪肝SULT2B1的表达是否受性类固醇调节。雄烯酮，睾丸激素和雌酮硫酸盐。该研究是在大白猪（40％）x种马（40％）x杜洛克（20％）杂种，平均car体重72.2千克的整个雄猪上进行的。研究表明，SULT2B1免疫反应蛋白表达可以被睾丸激素诱导（最终浓度为10和500 nM），而被硫酸雌酮抑制（最终浓度为100 nM）。在10 nM至1 oM的浓度范围内，雄烯酮对SULT2B1免疫反应蛋白的表达无明显影响。研究了类固醇作用的时程（0至48小时）。在类固醇治疗后24小时内观察到睾丸激素和硫酸雌酮的最大作用。这项研究提供直接的证据证明性类固醇参与猪肝SULTs的调控。

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