• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Livestock Science >Effects of soy-lecithin on lipid metabolism and hepatic expression of lipogenic genes in broiler chickens
【24h】

Effects of soy-lecithin on lipid metabolism and hepatic expression of lipogenic genes in broiler chickens

机译：大豆卵磷脂对肉仔鸡脂质代谢和脂肪形成基因肝表达的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The purpose of this experiment was to determine the effects of soy-lecithin on the lipid metabolism and expression of lipogenic genes in the liver of broiler chickens. Arbor Acres (AA) chicks growing from 1 to 42 days of age were randomly divided into 4 groups and fed corn-soybean basal diets containing 0% soy-lecithin (control), 0.5% soy-lecithin (SL1), 1% soy-lecithin (SL2), 2% soy-lecithin (SL3), respectively. At the end of experiment, samples of serum were taken for analyses of metabolites/hormones and liver tissue was collected to quantify expression of selected genes. The abdominal fat was removed and samples of thigh muscle and breast muscle also were collected. The hepatic expression of the genes encoding malic enzyme (ME), fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), sterol regulatory element binding protein-1 (SREBP-1), stearoyl-CoA (9) desaturase 1 (SCD1) and liver fatty acid binding protein (L-FABP) were determined with reverse transcription-polymerase chain reaction (RT-PCR) using SYBR green as a flourophore monitored in a real time mode. The study showed that the percentage of abdominal fat and liver fat were not significantly affected by soy-lecithin (P >0.05). SL2 group had the highest percentage of thigh muscle fat compared with other groups (P <0.05), but soy-lecithin had no significant effect on the percentage of breast muscle fat and the width of inter-muscular fat (P >0.05). Serum concentration of total serum cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) were reduced by soy-lecithin, whereas high-density lipoprotein cholesterol (HDL-C) and triglyceride (TG) were improved (P <0.05). The thyroid-stimulating hormone (TSH) and insulin (INS) were elevated in SL3 group (P <0.05). Furthermore, abundance of ME, FAS, ACC, SREBP-1, L-FABP and SCD1 mRNA were greater (P <0.05) in SL3 group. The results indicate that soy-lecithin alters the serum hormone levels and affects hepatic gene expression and thereby regulates fat metabolism of broilers.
机译：该实验的目的是确定大豆卵磷脂对肉鸡肝脏中脂质代谢和生脂基因表达的影响。将1至42天大的Arbor Acres（AA）小鸡随机分为4组，并喂食含0％大豆卵磷脂（对照），0.5％大豆卵磷脂（SL1），1％大豆卵磷脂的玉米-大豆基础日粮。卵磷脂（SL2），2％大豆卵磷脂（SL3）。在实验结束时，采集血清样品以分析代谢物/激素，并收集肝组织以量化所选基因的表达。去除腹部脂肪，并收集大腿肌肉和乳房肌肉的样本。编码苹果酸酶（ME），脂肪酸合酶（FAS），乙酰辅酶A羧化酶（ACC），固醇调节元件结合蛋白1（SREBP-1），硬脂酰辅酶A（9）去饱和酶1（ SCD1）和肝脂肪酸结合蛋白（L-FABP）是通过逆转录聚合酶链反应（RT-PCR）使用SYBR green作为荧光体以实时模式进行监测的。研究表明，大豆卵磷脂对腹部脂肪和肝脂肪的百分比没有显着影响（P> 0.05）。 SL2组的大腿肌肉脂肪百分比最高（P <0.05），而大豆卵磷脂对胸肌脂肪百分比和肌间脂肪宽度没有显着影响（P> 0.05）。大豆卵磷脂可降低血清总血清胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）的浓度，而高密度脂蛋白胆固醇（HDL-C）和甘油三酸酯（TG）则得到改善（P <0.05） 。 SL3组甲状腺刺激激素（TSH）和胰岛素（INS）升高（P <0.05）。此外，SL3组的ME，FAS，ACC，SREBP-1，L-FABP和SCD1 mRNA的丰度更高（P <0.05）。结果表明大豆卵磷脂改变血清激素水平并影响肝基因表达，从而调节肉鸡的脂肪代谢。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号