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首页> 外文期刊>Cellular immunology >The evaluation of survival and proliferation of lymphocytes in autologous mixed leukocyte reaction with dendritic cells. The comparison of incorporation of (3)H-thymidine and differential gating method.
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The evaluation of survival and proliferation of lymphocytes in autologous mixed leukocyte reaction with dendritic cells. The comparison of incorporation of (3)H-thymidine and differential gating method.

机译：评估自体混合性白细胞与树突状细胞反应中淋巴细胞的存活和增殖。 （3）H-胸腺嘧啶核苷掺入和微分门控方法的比较。

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摘要

Dendritic cells (DCs) play the key role in T-lymphocyte proliferation and induction of antitumour response. The mixed leukocyte reaction (MLR) of T-lymphocytes and DCs is essential instrument for immunological mechanisms studies. Conventionally used method for determination of T-lymphocytes proliferation, (3)H-thymidine incorporation, provides only general information. The method of flow cytometry and differential gating seems to be more suitable for quantitative and qualitative analysis of T-lymphocyte proliferation. It is based on time limited acquisition of events and on its distribution according to forward and side scatter values. We decided to compare these two methods and determine mutual correlation and compatibility. Eleven patients were studied and in all cases DCs promoted the survival and proliferation of both CD4 and CD8 lymphocytes. Both methods retained consistency with regard to survival and proliferation of CD4/CD8 lymphocytes. However, the correlation of these methods was not convincing. Therefore, both these methods might be used for evaluation of MLR, but each of them gives specific and complementary information.
机译：树突状细胞（DC）在T淋巴细胞增殖和诱导抗肿瘤反应中起关键作用。 T淋巴细胞和DC的混合白细胞反应（MLR）是进行免疫机制研究的重要工具。用于确定T淋巴细胞增殖的常规方法，（3）H-胸苷掺入仅提供一般信息。流式细胞仪和差异门控方法似乎更适合于T淋巴细胞增殖的定量和定性分析。它基于事件的时间限制获取以及根据事件的正向和侧向散布值的分布。我们决定比较这两种方法，并确定相互之间的相关性和兼容性。研究了11名患者，在所有情况下DC均促进CD4和CD8淋巴细胞的存活和增殖。两种方法在CD4 / CD8淋巴细胞的存活和增殖方面均保持一致。但是，这些方法的相关性没有说服力。因此，这两种方法都可以用于MLR的评估，但是每种方法都可以提供特定的补充信息。

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