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首页> 外文期刊>Radiology >MR imaging and scintigraphy of gene expression through melanin induction.
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MR imaging and scintigraphy of gene expression through melanin induction.

机译：MR成像和黑色素诱导基因表达闪烁显像。

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摘要

PURPOSE: To determine whether an expression vector that encodes for human tyrosinase, the key enzyme in the melanin production pathway, can be used to image gene expression with magnetic resonance (MR) imaging and scintigraphy. MATERIALS AND METHODS: Mouse fibroblasts and human embryonal kidney cells were transfected with an expression vector that contained a complete complementary DNA sequence that encodes the human tyrosinase gene (pcDNA3tyr). Transfected cells were assayed for messenger RNA presence, melanin staining, and indium-111 binding; scintigraphy and MR imaging were performed. RESULTS: Transfected cells contained tyrosinase messenger RNA and stained positively for melanin. Transfected cells had a higher In-111 binding capacity than nontransfected cells, a difference readily detectable with scintigraphy. MR imaging showed transfected cells to have markedly higher signal intensity after gene transfer than nontransfected cells. CONCLUSION: Gene transfer and expression in cell culture can be detected with MR imaging and scintigraphy. The proposed strategy of using an imaging marker gene may have a substantial effect on the noninvasive imaging of gene therapy.
机译：目的：确定编码人类酪氨酸酶（黑色素生成途径中的关键酶）的表达载体是否可用于通过磁共振（MR）成像和闪烁显像对基因表达进行成像。材料与方法：用表达载体转染小鼠成纤维细胞和人胚肾细胞，该表达载体包含编码人酪氨酸酶基因（pcDNA3tyr）的完整互补DNA序列。分析转染细胞的信使RNA的存在，黑色素染色和111铟的结合。进行了闪烁显像和磁共振成像。结果：转染的细胞含有酪氨酸酶信使RNA，黑色素呈阳性。转染的细胞比未转染的细胞具有更高的In-111结合能力，通过闪烁显色法可以轻松检测到差异。 MR成像显示转基因后的细胞比未转染的细胞具有明显更高的信号强度。结论：MR成像和闪烁显像可以检测细胞培养过程中的基因转移和表达。使用成像标记基因的拟议策略可能对基因治疗的非侵入性成像产生重大影响。

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