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首页> 外文期刊>Radiology >In vitro cytotoxicity of silver-impregnated collagen cuffs designed to decrease infection in tunneled catheters.
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In vitro cytotoxicity of silver-impregnated collagen cuffs designed to decrease infection in tunneled catheters.

机译：银浸渍的胶原袖带的体外细胞毒性旨在减少隧道导管中的感染。

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PURPOSE: To examine in vitro the effects of silver-impregnated collagen cuff material from central venous catheters on human fibroblast growth. MATERIALS AND METHODS: In culture flasks, hybrid cells were exposed to silver-impregnated collagen cuff material, and human fibroblasts were exposed to silver-impregnated or silver-free collagen cuff material. After 72 hours of growth, cells were stained and digitally imaged, and the relative areas of cytotoxicity were determined. RESULTS: Flasks containing the silver-impregnated collagen cuff material and hybrid cells or human fibroblasts showed a marked local cytotoxic effect of the cuff material; cell-free zones surrounding the cuff material were demonstrated. No cytotoxic effect was seen in the flasks that contained silver-free cuff material (control group). Mean area of cleared cells was 312 mm2 +/- 130 (range, 156-624 mm2) in the flasks containing human fibroblasts and silver-impregnated cuff material and 0 mm2 in the corresponding control flasks (P < .0001). Mean radius of the area of cleared cells around the silver-impregnated cuff material in the flasks containing human fibroblasts was 9.8 mm +/- 2.0 (range, 7.0-14.1 mm). CONCLUSION: Silver-impregnated collagen cuff material demonstrates a local cytotoxicity on hybrid cells and human fibroblasts in vitro. This finding may explain the phenomena seen clinically of decreased anchorage and inadvertent removal of catheters with silver-impregnated collagen cuffs.
机译：目的：在体外检查来自中央静脉导管的含银胶原袖带材料对人成纤维细胞生长的影响。材料与方法：在培养瓶中，将杂交细胞暴露于浸银的胶原套囊材料，将人类成纤维细胞暴露于浸银或无银的胶原囊套材料。生长72小时后，将细胞染色并进行数字成像，并确定细胞毒性的相对面积。结果：装有含银的胶原袖带材料和杂种细胞或人成纤维细胞的烧瓶显示袖带材料具有明显的局部细胞毒性作用;证实了袖带材料周围的无细胞区域。在包含无银袖套材料的烧瓶中（对照组）未观察到细胞毒性作用。在装有人成纤维细胞和浸有银的套囊材料的烧瓶中，澄清细胞的平均面积为312 mm2 +/- 130（范围为156-624 mm2），在相应的对照烧瓶中为0 mm2（P <.0001）。在包含人成纤维细胞的烧瓶中，银浸渍的袖带材料周围的透明细胞区域的平均半径为9.8 mm +/- 2.0（范围：7.0-14.1 mm）。结论：银浸渍的胶原袖带材料在体外对杂交细胞和人成纤维细胞显示出局部细胞毒性。这一发现可以解释临床上见到的现象：锚固减少和无意中注入了含银胶原袖带的导管。

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