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首页> 外文期刊>Cellular immunology >Nucleofection of a DNA vaccine into human monocyte-derived dendritic cells
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Nucleofection of a DNA vaccine into human monocyte-derived dendritic cells

机译：DNA疫苗对人单核细胞衍生的树突状细胞的核转染

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An efficient method for delivering DNA vaccines into dendritic cells is considered to be of paramount importance. Electroporation-based technology (nucleofection) has gained increasingly popularity, but few reports focused on the possible functional consequences related to this method. In this study, the nucleofection technique was used to transfer the recombinant plasmid into hMoDCs for phenotype expression analysis and immunopotency detection. The results showed that the nucleofection of increasing concentrations of plasmid DNA decreased the viability of the hMoDCs. The welfare of nucleofected hMoDCs depended on the dosage of the plasmid and the plasmid's retention time within the cells. Accompanied by the process of nucleofection, it would bring some non-specific changes. The methodology reported here is suggestive of a feasible system for DNA vaccine transfer into hMoDCs with the caution of certain undesired effect.
机译：将DNA疫苗递送至树突状细胞的有效方法被认为是最重要的。基于电穿孔的技术（核转染）已越来越受欢迎，但是很少有报道关注与该方法相关的可能功能后果。在这项研究中，使用核转染技术将重组质粒转移到hMoDC中，以进行表型表达分析和免疫力检测。结果表明，增加浓度的质粒DNA的核转染降低了hMoDC的活力。核转染的hMoDC的福利取决于质粒的剂量和质粒在细胞内的保留时间。伴随核转染过程，它将带来一些非特异性的变化。本文报道的方法学提示了将DNA疫苗转移到hMoDC中的可行系统，但要注意某些不良作用。

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