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首页> 外文期刊>Cellular immunology >Competitive binding of pentraxins and IgM to newly exposed epitopes on late apoptotic cells.
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Competitive binding of pentraxins and IgM to newly exposed epitopes on late apoptotic cells.

机译：五种毒素和IgM与晚期凋亡细胞上新暴露的表位竞争性结合。

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摘要

A random distribution of phospholipids among the inner and outer leaflet of the cell membrane occurs during apoptosis and is known as membrane flip-flop. Flip-flopped cells have binding sites for various plasma proteins, such as IgM and the pentraxins C-reactive protein (CRP) and serum amyloid P component (SAP). In this study, we investigated whether pentraxins and IgM antibodies recognize the same binding sites on apoptotic cells, and whether phospholipids constitute these binding sites. Except for SAP which also bound to early apoptotic cells, pentraxins and IgM preferentially bound to late apoptotic cells. Competition experiments with different phosphatemonoesters revealed that CRP and SAP as well as part of the IgM bound to the phospholipids head groups, SAP mainly to phosphorylethanolamine, CRP to phosphorylcholine and phosphorylethanolamine and to a lesser extent to phosphorylserine, and IgM to phosphorylcholine and phosphorylserine. These results were confirmed in experiments in which proteins were adsorbed from plasma with artificial phospholipids particles. IgM and the pentraxins variably competed for the same binding sites on late apoptotic cells, SAP having the highest and CRP the lowest apparent affinity. We conclude that CRP, SAP, and part of the IgM bind to the phospholipid head groups exposed on apoptotic cells. This shared specificity as well as their shared capability to activate complement, suggest that IgM and the pentraxins CRP and SAP exert similar functions in the removal of apoptotic cells.
机译：磷脂在细胞膜的内部和外部小叶之间随机分布，在细胞凋亡期间发生，被称为膜翻转。人字拖细胞具有与各种血浆蛋白（例如IgM和五味素C反应蛋白（CRP）和血清淀粉样蛋白P组分（SAP））结合的位点。在这项研究中，我们调查了pentraxins和IgM抗体是否识别凋亡细胞上的相同结合位点，以及磷脂是否构成这些结合位点。除了SAP也与早期凋亡细胞结合外，戊曲毒素和IgM优先与晚期凋亡细胞结合。不同磷酸单酯的竞争实验表明，CRP和SAP以及IgM的一部分与磷脂头基结合，SAP主要与磷酰乙醇胺结合，CRP与磷酰胆碱和磷酰乙醇胺结合，并在较小程度上与磷酰丝氨酸结合，IgM与磷酰胆碱和磷酰丝氨酸结合。这些结果在通过人工磷脂颗粒从血浆中吸附蛋白质的实验中得到了证实。 IgM和五种毒素在晚期凋亡细胞上竞争相同的结合位点，SAP的表观亲和力最高，而CRP的表观亲和力最低。我们得出结论，CRP，SAP和部分IgM与凋亡细胞上暴露的磷脂头基结合。这种共有的特异性以及它们激活补体的共有能力表明，IgM和五价毒素CRP和SAP在凋亡细胞的去除中具有相似的功能。

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