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首页> 外文期刊>Cellular immunology >Antigen-specific accumulation of naive, memory and effector CD4 T cells during anterior uveitis monitored by intravital microscopy.
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Antigen-specific accumulation of naive, memory and effector CD4 T cells during anterior uveitis monitored by intravital microscopy.

机译：活体显微镜检查前葡萄膜炎期间幼稚，记忆和效应CD4 T细胞的抗原特异性积累。

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摘要

Uveitis is an immune-mediated ocular disease and a leading cause of blindness. We characterized a novel model of uveitis with intravital microscopy. Transfer of ovalbumin-specific T cells from DO11.10 spleen to BALB/c recipients and subsequent challenge with ovalbumin in the anterior chamber of the eye resulted in anterior uveitis. Antigen-specificity was verified by injection of irrelevant antigen and transfer of T cells with a different specificity. Subsets of CD4 T cells, including naive (DO11.10 RAG(-/-)) and in vitro-activated Th2 effector CD4 T cells, infiltrated anterior segment tissues early in the inflammation. Memory-like CD44(high) CD4 T cells from unprimed transgenic mice and in vitro-activated Th1 effector CD4 T cells accumulated to larger numbers than naive or Th2 effector cells at 48 and 72 h. Of these, the alpha(2)-integrin+CD4 unprimed T cells entered the eye more efficiently, and antibody to alpha(2)-integrin markedly inhibited the inflammatory response. Intravital microscopy revealedthe early arrival and antigen-specific accumulation of CD4 T cells in inflamed tissue and should be helpful in understanding T cell migration to other organs.
机译：葡萄膜炎是免疫介导的眼病，也是失明的主要原因。我们表征了一种新型的葡萄膜炎模型与活体显微镜检查。卵白蛋白特异性T细胞从DO11.10脾脏转移至BALB / c受体，随后在眼前房中用卵白蛋白攻击，导致前葡萄膜炎。通过注射无关抗原和转移具有不同特异性的T细胞来验证抗原特异性。 CD4 T细胞亚群，包括幼稚的（DO11.10 RAG（-/-））和体外激活的Th2效应CD4 T细胞，在炎症的早期渗透到前节组织。来自未启动的转基因小鼠和体外激活的Th1效应CD4 T细胞的记忆样CD44（高）CD4 T细胞在48和72 h积累得比幼稚或Th2效应细胞大。其中，α（2）-整合素+ CD4未引发的T细胞更有效地进入眼睛，抗α（2）-整合素的抗体明显抑制了炎症反应。活体显微镜检查显示炎症组织中CD4 T细胞的早期到达和抗原特异性积聚，应该有助于理解T细胞向其他器官的迁移。

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