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首页> 外文期刊>Cellular immunology >Dendritic cells from HIV-1 infected individuals are less responsive to toll-like receptor (TLR) ligands
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Dendritic cells from HIV-1 infected individuals are less responsive to toll-like receptor (TLR) ligands

机译：来自HIV-1感染者的树突状细胞对通行费样受体（TLR）配体的反应较弱

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摘要

We compared TLR responsiveness in PBMC from HIV-1-infected and uninfected individuals using the TLR agonists: TLR7 (3M-001), TLR8 (3M-002), and TLR7/8 (3M-011). Activation and maturation of plasmacytoid dendritic cells (pDC) were measured by evaluating CD86, CD40, and CD83 expression and myeloid dendritic cell (mDC) activation was measured by evaluating CD40 expression. All agonists tested induced activation and maturation of pDC in PBMC cultures of cells from HIV+ and HIV- individuals. The TLR7 agonist induced significantly less pDC maturation in cells from HIV+ individuals. Quantitative assessment of secreted IFN-alpha and pro-inflammatory cytokines at the single cell level showed that pDC from HIV+ individuals stimulated with TLR7 and TLR7/8 induced IFN-alpha. TLR8 and TLR7/8 agonists induced IL-12 and COX-2 expression in mDC from HIV+ and HIV- individuals. Understanding pDC and mDC activation and maturation in HIV-1 infection could lead to more rational development of immunotherapeutic strategies to stimulate the adaptive immune response to HIV-1. (c) 2008 Elsevier Inc. All rights reserved.
机译：我们使用TLR7激动剂TLR7（3M-001），TLR8（3M-002）和TLR7 / 8（3M-011）比较了HIV-1感染者和未感染者的PBMC中TLR的反应能力。通过评估CD86，CD40和CD83表达来测量浆细胞样树突状细胞（pDC）的激活和成熟度，通过评估CD40表达来测量髓样树突状细胞（mDC）的激活作用。测试的所有激动剂在来自HIV +和HIV-个体的PBMC培养细胞中诱导了pDC的激活和成熟。 TLR7激动剂在HIV +个体的细胞中诱导的pDC成熟明显减少。在单细胞水平上对分泌的IFN-α和促炎性细胞因子的定量评估表明，用TLR7和TLR7 / 8刺激的HIV +个体的pDC诱导了IFN-α。 TLR8和TLR7 / 8激动剂在HIV +和HIV-个体的mDC中诱导IL-12和COX-2表达。了解pDC和mDC在HIV-1感染中的激活和成熟可能会导致更合理地发展免疫治疗策略，以刺激对HIV-1的适应性免疫反应。 （c）2008 Elsevier Inc.保留所有权利。

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