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High-throughput direct-infusion ion trap mass spectrometry: a new method for metabolomics

机译:高通量直接注入离子阱质谱法:一种新的代谢组学方法

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A fast method was developed to directly infuse raw plant extracts into a linear ion trap mass spectrometer, using the ion trap to isolate and fragment as many ions as possible from the extract. The full mass spectra can be analysed by multivariate statistics to determine discriminating ions, and the fragmentation data allows rapid classification or identification of these ions. The methodology was used to screen a wide range of strains of endophytic fungi in perennial ryegrass seeds for differences in metabolic profiles. The results show that this newly developed methodology is able to determine discriminating ions that can be present in very low concentrations. It also yielded sufficient fragmentation data to classify or identify the discriminating ions. Copyright (c) 2007 John Wiley & Sons, Ltd.
机译:开发了一种快速方法,可将原始植物提取物直接注入线性离子阱质谱仪中,使用离子阱从提取物中分离并破碎尽可能多的离子。可以通过多元统计分析全质谱,以确定可区分的离子,碎片数据可对这些离子进行快速分类或鉴定。该方法用于筛选多年生黑麦草种子中大量内生真菌菌株的代谢谱差异。结果表明,这种新开发的方法能够确定可能以极低浓度存在的区分离子。它还产生了足够的碎片数据来分类或识别区分离子。版权所有(c)2007 John Wiley&Sons,Ltd.

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