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首页> 外文期刊>Rapid Communications in Mass Spectrometry: RCM >Mass spectrometric study of glucose and cellobiose produced during enzymatic hydrolysis of alpha-cellulose extracted from oak late-wood annual rings
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Mass spectrometric study of glucose and cellobiose produced during enzymatic hydrolysis of alpha-cellulose extracted from oak late-wood annual rings

机译：从橡木晚材年轮中提取的α-纤维素酶水解过程中产生的葡萄糖和纤维二糖的质谱研究

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摘要

We present the first results concerning interannual variations in concentrations of glucose and cellobiose, obtained through enzymatic hydrolysis of alpha-cellulose. The alpha-cellulose was extracted from late-wood of oak. The tree-ring chronologies, wood components and their physical and chemical properties provide information about the ecosystem in which the tree grew, and thus information regarding climate variability and the impact of human activity in the past. The large molecular size and insolubility make it difficult to determine precisely the chemical and physical properties of the intact cellulose polymer. Enzymatic hydrolysis is the principal method of degradation of cellulose. In this study the feasibility has been examined of characterizing (alpha-cellulose through analysis by mass spectrometry (MS) of the degradation products from hydrolysis. Degradation of alpha-cellulose was possible without using alkaline or acid buffers. Analysis by MS provided the opportunity to obtain information on the biodegradation of saccharides. The presence of cellobiose and glucose in the degradation product was evidenced by the mass spectra. We have compared the abundances of these glucose and cellobiose ions with carbon isotope ratios, the efficiency of extraction of alpha-cellulose from the wood and tree-ring width indices. The challenge is to establish, with respect to climate changes and environmental conditions, the significance of the variations from one year to another in the observed abundances of glucose and cellobiose ions.
机译：我们提出有关葡萄糖和纤维二糖浓度的年际变化的第一个结果，这是通过酶水解α-纤维素获得的。 α-纤维素是从橡木的晚材中提取的。树木年轮的时间，木材成分及其物理和化学特性提供有关树木生长的生态系统的信息，从而提供有关气候多变性和过去人类活动影响的信息。大分子尺寸和不溶性使得难以精确确定完整纤维素聚合物的化学和物理性质。酶水解是纤维素降解的主要方法。在这项研究中，已经检验了表征（通过质谱分析水解水解产物的α-纤维素的可行性。无需使用碱或酸缓冲液即可降解α-纤维素。MS的分析提供了机会）获得有关糖类生物降解的信息，通过质谱证明降解产物中存在纤维二糖和葡萄糖，我们将这些葡萄糖和纤维二糖离子的丰度与碳同位素比，从中提取α-纤维素的效率进行了比较。挑战是要就气候变化和环境条件，确定观察到的葡萄糖和纤维二糖离子丰度从一年到另一年变化的重要性。

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