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首页> 外文期刊>Rapid prototyping journal >A novel sucrose porogen-based solid freeform fabrication system for bone scaffold manufacturing
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A novel sucrose porogen-based solid freeform fabrication system for bone scaffold manufacturing

机译：一种新型的基于蔗糖致孔剂的固体自由形式的骨骨架制造系统

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Purpose - Musculoskeletal conditions are a major health concern in the USA because of a large aging population and increased occurrence of sportrelated injuries. Bone tissue engineering may offer a less painful alternative to traditional bone grafts with lower risk of infection. The purpose of this paper is to present a novel porogen-based fabrication system for tissue engineering scaffolds using sucrose (C_(12)H_(22)O_(11)) as the porogen building material. Design/methodology/approach - A new solid freeform fabrication system has been developed and tested, which uses pressurized extrusion to print highly biocompatible and water soluble sucrose bone scaffold porogens (or negtives). Polycaprolactone (PCL) scaffolds are manufactured by injecting molten polymer into the porogens, and the porogens are subsequently dissolved with water. The resultant scaffolds demonstrate the defined porous structure designed into the sucrose porogen manufacturing computer-aided design model. Findings - To optimize the porogen manufacturing process, the viscosity of sucrose mixtures is measured. Design of experiments is used to plan and analyze the relationships between the porogen characteristics and the process parameters. Reservoir pressure and print head speed are identified as the dominant factors affecting sucrose flow rate and porogen strut diameter, respectively. The biocompatibility of the new system is assessed by in vitro cell culture testing. Endothelial hybridoma cells (EAhy 926) and osteoblasts (7F2) seeded on the fabricated PCL scaffolds adhered to the scaffold and proliferated over four to six days. Epifluorescence and scanning electron microscopy images reveal cell spreading and multiple layers of cells on the scaffold surface. The results demonstrated the potential of the structured sucrose porogen-based fabrication method in manufacturing bone tissue scaffolds. Originality/value - This paper describes the first time use of biomaterials-sucrose to make scaffold porogens and how an injection molded biopolymer scaffold can then be received.
机译：目的-由于人口老龄化和与运动有关的伤害增加，肌肉骨骼疾病是美国的主要健康问题。骨组织工程可以提供比传统的骨移植更痛苦的选择，并且具有较低的感染风险。本文的目的是提出一种使用蔗糖（C_（12）H_（22）O_（11））作为致孔剂建筑材料的，用于组织工程支架的基于致孔剂的新型制造系统。设计/方法/方法-已开发并测试了一种新型的固体自由形式制造系统，该系统使用加压挤压来印刷高度生物相容性和水溶性蔗糖骨支架成孔剂（或阴性物）。聚己内酯（PCL）支架是通过将熔融聚合物注入致孔剂中制成的，然后将致孔剂用水溶解。所得的支架展示了在蔗糖成孔剂制造计算机辅助设计模型中设计的确定的多孔结构。发现-为了优化成孔剂的生产过程，需要测量蔗糖混合物的粘度。实验设计用于计划和分析成孔剂特性与工艺参数之间的关系。储层压力和打印头速度分别被确定为影响蔗糖流速和成孔剂支撑直径的主要因素。通过体外细胞培养测试评估新系统的生物相容性。播种在制备的PCL支架上的内皮细胞杂交瘤细胞（EAhy 926）和成骨细胞（7F2）粘附在支架上，并在四到六天内增殖。落射荧光和扫描电子显微镜图像显示了支架表面上的细胞扩散和多层细胞。结果证明了基于结构化蔗糖成孔剂的制造方法在制造骨组织支架中的潜力。原创性/价值-本文介绍了首次使用生物材料蔗糖制造支架致孔剂，以及如何才能接收注射成型的生物聚合物支架。

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