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Characterization of PrPSc transmission from immune cells to neuronal cells

机译：PrPSc从免疫细胞向神经元细胞传播的表征

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We investigated PrPSc transmission in neuronal cells, spleen cells and several immune cells using an in vitro cell-to-cell transmission system. The transmission of PrPSc in the supernatant of PrPSc-infected neuronal cells was also investigated. We found that PrPSc transmission was more efficient in the cell-to-cell transmission system than in the supernatant-mediated system. PrPSc was more efficiently transmitted from adherent spleen cells to neuronal cells than from floating spleen cells. The adherent spleen cells were composed of macrophages (80%), dendritic cells (8%) and follicular dendritic cells (3%), indicating that macrophages play an important role in PrPSc transmission from immune cells to neuronal cells. Although PrPSc in the immune cells used as donor cells was gradually degraded, the PrPSc transmitted to neuronal cells was observed by Western blot analysis. Investigation of the mechanism of PrPSc transmission between cells represents an important step towards understanding the pathogenesis of prion diseases.
机译：我们使用体外细胞间传输系统研究了PrPSc在神经元细胞，脾细胞和几种免疫细胞中的传播。还研究了PrPSc在感染PrPSc的神经元细胞上清液中的传播。我们发现，PrPSc传输在细胞间传输系统中比在上清液介导的系统中更有效。 PrPSc从粘附的脾细胞比从漂浮的脾细胞更有效地传播到神经元细胞。粘附的脾细胞由巨噬细胞（80％），树突状细胞（8％）和滤泡树突状细胞（3％）组成，表明巨噬细胞在PrPSc从免疫细胞向神经元细胞的传递中起重要作用。尽管用作供体细胞的免疫细胞中的PrPSc逐渐降解，但通过蛋白质印迹分析观察到了传递至神经元细胞的PrPSc。对PrPSc在细胞之间传播的机制的研究代表了了解understanding病毒疾病发病机理的重要一步。

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