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首页> 外文期刊>Cell communication & adhesion >Gene Printer: Laser-Scanning Targeted Transfection of Cultured Cardiac Neonatal Rat Cells
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Gene Printer: Laser-Scanning Targeted Transfection of Cultured Cardiac Neonatal Rat Cells

机译：基因打印机：激光扫描培养的心脏新生大鼠细胞的靶向转染。

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摘要

Heterogeneous gene expression in cardiac cells and tissues which requires targeted delivery of foreign DNA into selected cells or regions is needed for the development of novel therapies. Several techniques have been employed for targeted transfection, such as direct microinjection into cells or targeted electroporation. However, these techniques have limited bandwidth or spatial resolution of transfection. We aimed to develop a method for transfection of cardiac cells by means of laser-assisted optoporation using a standard confocal microscope. This technique allows for the transfection of selected cell types in the presence of other cell types as long as they are distinguishable with a microscope. This technique can work as a “gene printer” creating arbitrarily shaped areas of transfected cells.
机译：开发新型疗法需要在心脏细胞和组织中异源基因表达，这需要将外源DNA靶向递送到选定的细胞或区域中。几种技术已用于靶向转染，例如直接显微注射到细胞中或靶向电穿孔。但是，这些技术的转染带宽或空间分辨率有限。我们旨在开发一种通过使用标准共聚焦显微镜进行激光辅助的对位作用转染心肌细胞的方法。该技术允许在存在其他细胞类型的情况下转染选定的细胞类型，只要它们在显微镜下即可区分。该技术可以用作“基因打印机”，以创建任意形状的转染细胞区域。

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