• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell communication & adhesion >Gap Junctional Communication Does not Contribute to the Interaction Between Neutrophils and Airway Epithelial Cells
【24h】

Gap Junctional Communication Does not Contribute to the Interaction Between Neutrophils and Airway Epithelial Cells

机译：间隙连接通讯不有助于嗜中性粒细胞和气道上皮细胞之间的相互作用。

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cystic fibrosis (CF) is characterized by intense neutrophil migration into the airways. Increasing evidence indicates that interaction between neutrophils and airway epithelial cells contributes to the modulation of the inflammatory response. Blood neutrophils were reported to express connexins and form gap junctions with endothelial cells, thereby establishing gap junctional communication. We tested whether altered communication between human neutrophils and airway epithelial cells may contribute to the exaggerated inflammatory response observed in CF patients. Microinjections did not reveal dye coupling between activated blood neutrophils. By constrast, diffusion of calcein between neutrophils and airway epithelial cells of CF or non-CF origin was observed in transmigration and adhesion assays. This diffusion was prevented with probenicid, an inhibitor of ATP-dependent organic anion pumps, but not with gap junction blockers. Finally, RT-PCR failed to detect mRNAs for six connexins in blood neutrophils. These results suggest that gap junctional communication does not contribute to neutrophil-airway epithelial cell interaction.
机译：囊性纤维化（CF）的特征是嗜中性粒细胞大量迁移到气道中。越来越多的证据表明，嗜中性粒细胞和气道上皮细胞之间的相互作用有助于炎症反应的调节。据报道，血液中性粒细胞表达连接蛋白并与内皮细胞形成间隙连接，从而建立间隙连接通讯。我们测试了人类嗜中性粒细胞和气道上皮细胞之间交流的改变是否可能导致CF患者的炎症反应过度。显微注射未显示活化血液中性粒细胞之间的染料偶联。相比之下，钙黄绿素在中性粒细胞和CF或非CF来源的气道上皮细胞之间的扩散在转运和粘附试验中观察到。这种扩散是通过丙二酸（一种依赖ATP的有机阴离子泵的抑制剂）阻止的，但没有用间隙连接阻滞剂阻止。最后，RT-PCR无法检测血液中性粒细胞中六种连接蛋白的mRNA。这些结果表明，间隙连接通讯不有助于中性粒细胞-气道上皮细胞的相互作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号