v-Src Tyrosine Phosphorylation of Connexin43: Regulation of Gap Junction Communication and Effects on Cell Transformation

Rui Lin; Kendra D. Martyn; Carrie V. Guyette; Alan F. Lau; Bonnie J. Warn-Cramer

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v-Src Tyrosine Phosphorylation of Connexin43: Regulation of Gap Junction Communication and Effects on Cell Transformation

机译：连接蛋白的v-Src酪氨酸磷酸化：间隙连接通讯的调节及其对细胞转化的影响

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The oncogenic tyrosine kinase, v-Src, phosphorylates connexin43 (Cx43) on Y247 and Y265 and inhibits Cx43 gap junctional communication (GJC), the process of intercellular exchange of ions and metabolites. To test the role of a negative charge on Cx43 induced by tyrosine phosphorylation, we expressed Cx43 with glutamic acid substitutions at Y247 or Y265. The Cx43Y247E or Cx43Y265E channels were functional in Cx43 knockout fibroblasts, indicating that introducing a negative charge on Cx43 was not likely the mechanism for v-Src disruption of GJC. Cells coexpressing v-Src and the triple serine to alanine mutant, Cx43S255/279/282A, confirmed that mitogen-activated protein (MAP) kinase phosphorylation of Cx43 was not required for v-Src-induced disruption of GJC and that tyrosine phosphorylation was sufficient. In addition, v-Src cells containing v-Src-resistant gap junctions, Cx43Y247/265F, displayed properties of cell migration, adhesion, and proliferation similar to Cx43wt/v-Src cells, suggesting that Cx43 tyrosine phosphorylation and disruption of GJC are not involved in these transformed cell properties.

机译：致癌酪氨酸激酶v-Src使Y247和Y265上的connexin43（Cx43）磷酸化，并抑制Cx43间隙连接通讯（GJC），离子和代谢物的细胞间交换过程。为了测试酪氨酸磷酸化诱导的Cx43负电荷的作用，我们在Y247或Y265处表达了带有谷氨酸取代的Cx43。 Cx43Y247E或Cx43Y265E通道在Cx43基因敲除的成纤维细胞中起作用，这表明在Cx43上引入负电荷不太可能是v-Src破坏GJC的机制。共表达v-Src和三丝氨酸至丙氨酸突变体Cx43S255 / 279 / 282A的细胞证实，v-Src诱导的GJC破坏不需要Cx43的促分裂原活化蛋白（MAP）激酶磷酸化，酪氨酸磷酸化就足够了。此外，包含v-Src抵抗性缺口连接的v-Src细胞Cx43Y247 / 265F与Cx43wt / v-Src细胞相似，显示出细胞迁移，粘附和增殖的特性，这表明Cx43酪氨酸磷酸化和GJC破坏没有参与这些转化的细胞特性。

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《Cell communication & adhesion》 |2006年第4期|共18页
作者
Rui Lin; Kendra D. Martyn; Carrie V. Guyette; Alan F. Lau; Bonnie J. Warn-Cramer;
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正文语种 eng
中图分类细胞生物学;
关键词
Connexin43; gap junction communication; v-Src; phosphorylation; transformation; MAP kinase;

机译：连接蛋白43;间隙连接通讯;v-Src;磷酸化;转化;MAP激酶;

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1. v-Src Tyrosine Phosphorylation of Connexin43: Regulation of Gap Junction Communication and Effects on Cell Transformation [J] . Rui Lin, Kendra D. Martyn, Carrie V. Guyette, Cell communication & adhesion . 2006,第4期

机译：连接蛋白的v-Src酪氨酸磷酸化：间隙连接通讯的调节及其对细胞转化的影响
2. Maintaining Connexin43 Gap Junctional Communication in v-Src Cells Does Not Alter Growth Properties Associated with the Transformed Phenotype [J] . Bonnie J. Warn-Cramer, Rui Lin, Kendra Martyn, Cell communication & adhesion . 2003,第4a6期

机译：在v-Src细胞中维持Connexin43间隙连接通讯不会改变与转化表型相关的生长特性
3. Tyrosine phosphorylation of the gap junction protein connexin43 is required for the pp60v-src-induced inhibition of communication. [J] . K I Swenson, H Piwnica-Worms, H McNamee, Cell Regulation . 1990,第13期

机译：间隙连接蛋白连接蛋白43的酪氨酸磷酸化是pp60v-src诱导的通讯抑制所必需的。
4. Effects of surface functional groups on interacted-cell behavior: Effect on gap-junctional intercellular communication of cells [C] . Ryusuke Nakaoka, Atsuko Matsuoka World biomaterials congress . 2012

机译：表面官能团对相互作用细胞行为的影响：对间隙连接的细胞间通讯的影响。
5. Mitogen-activated protein kinase-induced phosphorylation of cardiac gap junctions and regulation of the intercellular communication after ischemia. [D] . Cameron, Scott James. 2003

机译：丝裂素活化的蛋白激酶诱导的心脏间隙连接的磷酸化和缺血后细胞间通讯的调节。
6. v-Src Tyrosine Phosphorylation of Connexin43: Regulation of Gap Junction Communication and Effects on Cell Transformation [O] . Rui Lin, Kendra D. Martyn, Carrie V. Guyette, -1

机译：连接蛋白的v-Src酪氨酸磷酸化：间隙连接通讯的调节及其对细胞转化的影响
7. A characterization of permolybdate and its effect on cellular tyrosine phosphorylation, gap junctional intercellular communication and phosphorylation status of the gap junction protein, connexin43 [O] . Mikalsen Svein-Ole, Kaalhus Olav 1997

机译：高钼酸盐的表征及其对细胞酪氨酸磷酸化，间隙连接细胞间通讯和间隙连接蛋白connexin43的磷酸化状态的影响

v-Src Tyrosine Phosphorylation of Connexin43: Regulation of Gap Junction Communication and Effects on Cell Transformation

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