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首页> 外文期刊>Reproduction in Domestic Animals >Effect of Varying Glucose Concentrations during In Vitro Maturation and Embryo Culture on Efficiency of In Vitro Embryo Production in Buffalo
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Effect of Varying Glucose Concentrations during In Vitro Maturation and Embryo Culture on Efficiency of In Vitro Embryo Production in Buffalo

机译：水牛体外成熟和胚培养过程中变化的葡萄糖浓度对体外胚产生效率的影响

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摘要

This study was aimed to optimize glucose level at different stages of buffalo in vitro embryo production procedure. Three glucose levels (1.5, 5.6 and 10 mm) along with a control (0 mm) were used at three phases of in vitro fertilisation (IVF) procedure viz. in vitro maturation (IVM), in vitro culture (IVC-I) (1272 hpi) and IVC-II (72 hpi to 7 dpi). Maturation rate of oocytes was found different under different glucose concentrations, and significantly more number of oocytes reached to MII under 5.6 mm glucose. The glucose levels at each phase (IVM, IVC-I and IVC-II) individually had significant effect on blastocyst rate, and the level used at one phase had significant effect on the outcome of next phase. Complete withdrawal of glucose from any of these stages irrespective of concentrations used at subsequent stage/s resulted in significantly lower number of blastocysts. However, the changing levels of glucose had differential effects during different phases of IVF steps. The most prominent effect of glucose level was observed during IVM. The presence of 5.6 mm glucose at all stages was most effective to yield highest blastocyst rate in buffalo IVF system.
机译：这项研究旨在优化水牛体外胚胎生产过程中不同阶段的葡萄糖水平。在体外受精（IVF）过程的三个阶段使用三个葡萄糖水平（1.5、5.6和10 mm）以及一个对照（0 mm）。体外成熟（IVM），体外培养（IVC-I）（1272 hpi）和IVC-II（72 hpi至7 dpi）。发现在不同的葡萄糖浓度下卵母细胞的成熟率不同，并且在5.6mm的葡萄糖下达到MII的卵母细胞数目明显增加。每个阶段（IVM，IVC-I和IVC-II）的葡萄糖水平分别对胚泡率有显着影响，并且在一个阶段使用的水平对下一阶段的结局具有显着影响。无论随后阶段使用的浓度如何，从这些阶段中的任何一个阶段完全抽出葡萄糖都会导致胚泡的数量大大减少。但是，葡萄糖水平的变化在体外受精步骤的不同阶段有不同的影响。在IVM期间观察到葡萄糖水平最显着的作用。在所有阶段，在水牛IVF系统中，存在5.6毫米葡萄糖最能产生最高的胚泡率。

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