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Influence of Systematically Varied Nano-Scale Topography on Cell Morphology and Adhesion

机译：系统变化的纳米尺度地形对细胞形态和粘附的影响

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摘要

The types of cell-matrix adhesions and the signals they transduce strongly affect the cell-phenotype. We hypothesized that cells sense and respond to the three-dimensionality of their environment, which could be modulated by nano-structures on silicon surfaces. Human foreskin fibroblasts were cultured on nano-structures with different patterns (nano-post and nano-grate) and heights for 3 days. The presence of integrin 5, 1, 3, paxillin and phosphorylated FAK (pFAK) were detected by western blot and immunofluorescence. Integrin 3 exhibited stronger signals on nano-grates. pFAK and paxillin were observed as small dot-like patterns on the cell-periphery on nano-posts and as elongated and aligned patterns on nano-grates. Collectively, our observations highlighted the presence of focal (integrin 1, 3, pFAK, paxillin), fibrillar (integrin 5, 1) and 3-D matrix (integrin 5, 1, paxillin) adhesions on nano-structures. The presented nano-structures offer interesting opportunities to study the interaction of cells with topographical features comparable to the size of extracellular matrix components.
机译：细胞-基质粘附的类型及其传递的信号强烈影响细胞表型。我们假设细胞可以感知并响应其环境的三维，而三维可以通过硅表面上的纳米结构进行调节。将人包皮成纤维细胞在具有不同图案（纳米柱和纳米格栅）和高度的纳米结构上培养3天。通过蛋白质印迹和免疫荧光检测整合素5、1、3，paxillin和磷酸化FAK（pFAK）的存在。整联蛋白3在纳米格栅上表现出更强的信号。 pFAK和paxillin在纳米柱上的细胞周围观察为小点状图案，在纳米格栅上观察为细长和排列的图案。总的来说，我们的观察结果强调了纳米结构上存在粘着斑（整合素1、3，pFAK，paxillin），原纤维（整合素5、1）和3-D基质（整合素5、1，paxillin）的粘附。提出的纳米结构提供了有趣的机会来研究具有地形特征的细胞相互作用，这些特征与细胞外基质成分的大小相当。

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