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首页> 外文期刊>Research Journal of Chemistry and Environment >Remediation of TCE-eontaminated groundwater using integrated biosparging and enhanced bioremediation system
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Remediation of TCE-eontaminated groundwater using integrated biosparging and enhanced bioremediation system

机译：使用集成的生物喷射和增强型生物修复系统修复受TCE污染的地下水

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A biosparging well (BSW) was installed at the up gradient area of the test site for air injection. Brown sugar, which was used as the primary substrate, was pressure-injected into the TCEplume through the BSW to enhance the rate of TCE co-metabolism.To evaluate the effectiveness of TCE bioremediation, monitor wells were installed in series down gradient of the BSW along the groundwater flow path. Polymerase chain reaction (PCR) and nucleotide sequence analyses indicate that TCE-degrading enzymes (e.g. toluene mono oxygenase, toluene dioxygenase, phenol mono-oxygenase and particulate methane monooxygenase) were identified in field groundwater samples after the air and substrate supplement. Results from the pilot-scale study indicate that the aerobicco-metabolism was the major cause of the decrease in TCE concentrations. Without primary substrate supplement, intrinsic bioremediation and injection of air and nutrients alone could not enhance the aerobic co-metabolic mechanism and cause the decreasein TCE concentrations within the plume. The occurrence of aerobic TCE co-metabolism could be confirmed by the following investigations within the plume: (1) significant decrease in TCE concentrations; (2) increase in chemical oxygen demand (COD) concentrations and microbial populations; (3) increase in dissolved oxygen (DO) and oxidation-reduction potential (ORP) in the BSW; (4) significant depletion of DO and decrease in ORP after the supplement of substrate; (5) production of CO_2 and decrease in pH value and (6) detected specific TCE-degrading genes. Approximately 97 and 92% of TCE was removed in BSW and monitor well located 5 m down gradient of the BSW. The operation of biosparging caused the shifting of low oxygen conditions inside the plume to aerobic conditions. Results indicate that the integrated in situ biosparging and enhanced aerobic bioremediation is a feasible and promising technology to remediate TCE-contaminated groundwater.
机译：在测试现场的向上坡度区域安装了一个生物喷射井（BSW），用于空气注入。通过BSW将黑糖用作主要底物，将其通过BSW压力注入TCEplume中，以提高TCE共代谢的速率。为评估TCE生物修复的有效性，在BSW的向下梯度安装了监测孔沿地下水流动路径。聚合酶链反应（PCR）和核苷酸序列分析表明，在补充空气和底物后，在现场地下水样品中鉴定出了TCE降解酶（例如甲苯单加氧酶，甲苯双加氧酶，苯酚单加氧酶和颗粒甲烷单加氧酶）。中试研究的结果表明，好氧代谢是TCE浓度降低的主要原因。如果没有主要的底物补充剂，仅进行固有的生物修复以及仅注入空气和营养就无法增强有氧代谢机制，并导致羽状流中TCE浓度降低。有氧的TCE共代谢的发生可以通过羽内的以下研究证实：（1）TCE浓度显着下降; （2）增加化学需氧量（COD）浓度和微生物种群; （3）BSW中溶解氧（DO）和氧化还原电位（ORP）的增加; （4）底物添加后，DO大量消耗，ORP降低; （5）CO_2的产生和pH值的降低;（6）检测到特定的TCE降解基因。在BSW中约有97％和92％的TCE被去除，并监测BSW向下5 m处的井眼。生物喷雾的操作导致烟羽内部的低氧条件转变为有氧条件。结果表明，整合原位生物喷射和加强好氧生物修复是修复受TCE污染的地下水的可行和有前途的技术。

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