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首页> 外文期刊>Cell cycle >Snf1/AMPK promotes S-phase entrance by controlling CLB5 transcription in budding yeast.
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Snf1/AMPK promotes S-phase entrance by controlling CLB5 transcription in budding yeast.

机译：Snf1 / AMPK通过控制发芽酵母中的CLB5转录来促进S期进入。

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摘要

The Saccharomyces cerevisiae Snf1 protein kinase has been reported to be required for adaptation to glucose limitation and for growth on non-fermentable carbon sources. Here we present novel findings indicating that Snf1, the key regulator of cellular energy, is also involved in yeast cell cycle control. The lack of Snf1 alpha-catalytic subunit downregulates the growth rate and CLB5 expression, delaying Sld2 phosphorylation and G(1)/S transition, in cells grown in 2%, but not in 5% glucose. A non-phosphorylatable Snf1 rescues the slow growth phenotype, whereas a wild type or a phosphomimetic mutant is required to rescue growth rate and the G(1)/S delay. Using either Snf1 or Swi6 as a bait, a specific interaction of Snf1 with Swi6, the regulatory subunit of MBF, was detected. In conclusion, this report describes a previously unrecognized role for Snf1 in transcriptional modulation of the G(1) to S transition, differing from the reported AMPK role in controlling the G(1)/S transition in multicellular eukaryotes.
机译：据报道，酿酒酵母Snf1蛋白激酶是适应葡萄糖限制和在非发酵碳源上生长所必需的。在这里，我们提出新颖的发现，表明细胞能量的关键调节因子Snf1也参与了酵母细胞周期的控制。 Snf1α催化亚基的缺乏下调生长速度和CLB5表达，延迟Sld2磷酸化和G（1）/ S过渡，在2％，但不是在5％葡萄糖中生长的细胞。不可磷酸化的Snf1可以挽救缓慢的生长表型，而野生型或拟膦酸突变体则可以挽救生长速率和G（1）/ S延迟。使用Snf1或Swi6作为诱饵，检测到Snf1与Swi6（MBF的调节亚基）的特异性相互作用。总之，此报告描述了Snf1在G（1）向S过渡的转录调节中的转录功能，这一作用与以前报道的AMPK在多细胞真核生物中控制G（1）/ S过渡的作用不同。

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