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Is there a 'G spot' in sister chromatid cohesion resolution?

机译：姐妹染色单体内聚力分辨率中是否有“ G点”？

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GTPases are a well-known family of proteins involved in a wide variety of crucial regulatory processes in the cell. A structurally distinct and highly conserved subfamily of GTPases called GPN-loop GTPases has recently been described in archae and eukaryotes. The new family of G proteins is characterized by a conserved Gly-Pro-Asn motif inserted onto the GTPase core-fold. The new study by Alonso et al. published in the June 1 st issue of Cell Cycle provides evidence that the GPN-loop GTPase, yGPN1, is involved in the regulation of sister chromatid resolution in yeast cells.The authors used S. cerevisiae strains in which the endogenous copy of yGPNI was deleted by homologous recombination, and yGPNI expression was reconstituted by a rescue plasmid coding for yGPNI under the control of an inducible promoter. In the absence of yGPNI, there was a decrease of the growth rate, and the majority of the cells had an unusually large bud. Flow cytometric analysis showed a severe S-phase delay in asynchro-nously cycling cells under yGPNI repression as well as in cells allowed to proceed to the next cell cycle following mitotic synchronization. Compared to wild-type cells, microscopic analysis of cells repressed for yGPNI at G_1/M phase revealed a greater number of doublets, with a large bud containing a nucleus whose position is adjacent to the neck or whose nucleus is passing from mother to daughter cell. In contrast, induction of yGPNI expression resulted in an increase in the proportion of cells with a large bud containing one nucleus each.
机译：GTPases是一个众所周知的蛋白质家族，涉及细胞中各种关键的调控过程。最近在古生物和真核生物中描述了一个结构独特且高度保守的GTPases亚家族，称为GPN环GTPases。 G蛋白新家族的特征是在GTPase核心折叠上插入了保守的Gly-Pro-Asn基序。阿隆索等人的新研究。发表在6月1日《细胞周期》上的文章提供了证据，表明GPN环GTP酶yGPN1参与了酵母细胞中姐妹染色单体解析的调控。作者使用了酿酒酵母菌株，其中删除了yGPNI的内源性拷贝通过同源重组，在诱导型启动子的控制下，通过编码yGPNI的救援质粒重建yGPNI的表达。在没有yGPNI的情况下，生长速率降低，并且大多数细胞具有异常大的芽。流式细胞仪分析显示，在yGPNI抑制下，非周期循环细胞以及有丝分裂同步后可进入下一细胞周期的细胞存在严重的S期延迟。与野生型细胞相比，对在G_1 / M期受yGPNI抑制的细胞进行显微镜分析显示，其双联体数量更多，其中大芽含有一个核，其位置与颈部相邻或其核从母体传给子代细胞。 。相反，yGPNI表达的诱导导致具有大芽的细胞比例增加，每个芽含有一个核。

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