• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Reproduction in Domestic Animals >In Vitro Maturation and Development of Porcine Oocytes Cultured in a Straw or Dish Using a Portable Incubator with a CO2 Chamber
【24h】

In Vitro Maturation and Development of Porcine Oocytes Cultured in a Straw or Dish Using a Portable Incubator with a CO2 Chamber

机译：使用带有CO2箱的便携式培养箱在稻草或培养皿中培养的猪卵母细胞体外成熟和发育

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We investigated the effects of a portable incubator with a CO2 chamber on the viability and development of porcine oocytes/embryos for their transportation and examined the operational suitability of a straw or dish as a container for culturing the oocytes or embryos in the portable incubator. In the first experiment, the cumulus-oocyte complexes (COCs) were placed either in a dish or straw; and they were then cultured for 44 h in a standard CO2 incubator, in the CO2 chamber in an incubator, or in the CO2 chamber in a portable incubator. The matured oocytes were fertilized with frozen-thawed spermatozoa and then cultured in a dish in the standard CO2 incubator for 8 days. There were no differences in the proportions of oocytes reaching metaphase II stage among the groups. However, the proportions of cleavage and development to blastocysts derived from oocytes matured in a straw were lower than those from oocytes matured in a dish, irrespective of the type of incubator used. In the second experiment, the COCs were matured in a dish in the standard CO2 incubator, and the matured oocytes were fertilized and then placed either in a dish or straw. These were then cultured for 8 days in the standard CO2 incubator or portable incubator. Some zygotes cultured in the portable incubator developed to the blastocyst stage. The proportions of cleavage and development to blastocysts were significantly lower for putative zygotes cultured in straw than for those cultured in dish, irrespective of the type of incubator used. Our results indicate that a portable incubator with a CO2 chamber can maintain the viability and development of oocytes/embryos, but the straw is not a suitable system for in vitro culture of the oocytes/embryos during transportation.
机译：我们研究了带有CO2腔室的便携式孵化器对猪卵母细胞/胚胎运输和运输的生存能力和发育的影响，并研究了将稻草或碟子用作在便携式孵化器中培养卵母细胞或胚胎的容器的操作适用性。在第一个实验中，将卵-卵母细胞复合物（COC）放在盘子或稻草中。然后将它们在标准的CO2培养箱中，培养箱中的CO2箱中或便携式培养箱中的CO2箱中培养44小时。用冷冻融化的精子使成熟的卵母细胞受精，然后在标准CO2培养箱中的培养皿中培养8天。各组之间达到II期中期的卵母细胞比例没有差异。然而，无论使用哪种培养箱，在吸管中成熟的卵母细胞对卵囊的卵裂和发育的比例都比在盘中成熟的卵母细胞的卵裂和发育的比例低。在第二个实验中，将COC在标准CO2恒温箱中的培养皿中成熟，然后将成熟的卵母细胞受精，然后放入培养皿或吸管中。然后将它们在标准CO2培养箱或便携式培养箱中培养8天。在便携式培养箱中培养的一些受精卵发育到了胚泡期。不论使用哪种培养箱，在稻草中培养的推定合子的裂解和发育比例均显着低于在碟中培养的合子。我们的结果表明，带有CO2腔室的便携式培养箱可以保持卵母细胞/胚胎的生存能力和发育，但是秸秆并不是运输过程中卵母细胞/胚胎体外培养的合适系统。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号