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首页> 外文期刊>Research communications in molecular pathology and pharmacology >Enhanced generation of leukotriene B4 and superoxide radical from calcium ionophore (A23187) stimulated human neutrophils after priming with interferon-alpha.
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Enhanced generation of leukotriene B4 and superoxide radical from calcium ionophore (A23187) stimulated human neutrophils after priming with interferon-alpha.

机译：用干扰素-α引发后，钙离子载体（A23187）产生的白三烯B4和超氧化物自由基的产生增强，刺激了人类中性粒细胞。

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摘要

The present study was designed to investigate the effect of interferon-alpha ( INF-alpha) on the production of leukotrienes (LTs) and superoxide radicals when intact human neutrophils were stimulated with calcium ionophore (A23187). A reverse phase high performance liquid chromatography and UV spectroscopy were employed to detect and quantitate the released LTs; namely LTC4, LTB4 and its trans isomers, 6-trans LTB4 and 12-epi-6-trans LTB4. Preincubation of intact human neutrophils at 37 degrees C for 30 min with INF-alpha and stimulation with calcium ionophore A23187 for 1 min enhanced significantly the formation of LTB4. Preincubation of intact human neutrophils with INF-alpha and subsequent stimulation with calcium ionophore A23187 also enhanced significantly superoxide radical generation that reduced nitroblue tetrazolium into blue formazan. The in vivo effect of INF-alpha in rats demonstrated that the higher dose of INF-alpha that induced superoxide radical and LTB4 by A23187 stimulated intact human neutrophil in vitro, also induced a significant decrease in white blood cells and RBCs started at 4 h after i.p administration. The differential white blood cell count revealed that, the prime target for INF-alpha is the white blood cells of myeloid origin. These results might demonstrate the modulatory effects of INF-alpha on granulocyte functions.
机译：本研究旨在研究当钙离子载体（A23187）刺激完整的人类嗜中性粒细胞时，干扰素-α（INF-α）对白三烯（LTs）和超氧化物自由基产生的影响。采用反相高效液相色谱和紫外光谱法检测和定量释放的LT。即LTC4，LTB4及其反式异构体，即6反式LTB4和12-epi-6反式LTB4。完整的人类嗜中性粒细胞与INF-alpha在37摄氏度下预孵育30分钟，并用钙离子载体A23187刺激1分钟，显着增强了LTB4的形成。完整人类嗜中性粒细胞与INF-α的预温育以及随后钙离子载体A23187的刺激也显着增强了超氧自由基的产生，从而使硝基蓝四唑还原为蓝色甲an。 INF-α对大鼠的体内作用表明，较高剂量的INF-α会在A4187体外诱导超氧自由基和LTB4刺激完整的人嗜中性粒细胞，还诱导白细胞和RBC的显着减少始于4 h ip管理。白细胞计数差异表明，INF-α的主要靶标是髓样来源的白细胞。这些结果可能证明INF-α对粒细胞功能的调节作用。

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