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首页> 外文期刊>Research communications in molecular pathology and pharmacology >Determination of a new asiatic acid derivative, AS 2-006A in rat plasma and urine, and human plasma by high-performance liquid chromatography.
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Determination of a new asiatic acid derivative, AS 2-006A in rat plasma and urine, and human plasma by high-performance liquid chromatography.

机译：高效液相色谱法测定大鼠血浆和尿液以及人血浆中的一种新的积雪草酸衍生物AS 2-006A。

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摘要

A high-performance liquid chromatographic method was developed for the determination of a new wound healing agent, AS 2-006A (ethoxymethyl 2-oxo-3, 23-O-isopropylideneasiatate), in rat plasma and urine, and human plasma. The sample preparation was simple: 2 volumes of acetonitrile were added to the biological samples to deproteinize it. A 50-microl aliquot of the supernatant was injected onto the reversed-phase column. The mobile phase employed was acetonitrile : H2O (9:1, v/v) and run at a flow rate of 1.1 ml/min. The column effluent was monitored by a UV detector set at 205 nm. The retention time for AS 2-006A was approximately 29.5 min. The detection limits for AS 2-006A in rat and human plasma were both 1 microg/ml, and in rat urine was 2 microg/ml. The coefficients of variation of the assay (within-day and between-day) were generally low (below 10.8%) for rat plasma and urine, and human plasma. No interferences from endogenous substances were found.
机译：开发了一种高效液相色谱方法，用于测定大鼠血浆和尿液以及人体血浆中的新型伤口愈合剂AS 2-006A（乙氧基甲基2-oxo-3、23-O-异丙基亚甲基磺酸盐）。样品制备很简单：将2体积的乙腈添加到生物样品中以使其脱蛋白。将50微升等分试样的上清液注入反相色谱柱。所使用的流动相为乙腈：H2O（9：1，v / v），流速为1.1 ml / min。通过设置在205 nm的UV检测器监控柱流出物。 AS 2-006A的保留时间约为29.5分钟。大鼠和人血浆中AS 2-006A的检出限均为1微克/毫升，大鼠尿中的检出限为2微克/毫升。对于大鼠血浆，尿液和人血浆，测定的变异系数（日内和日间）通常较低（低于10.8％）。没有发现来自内源性物质的干扰。

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