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首页> 外文期刊>Research in Veterinary Science >Oral inoculation of neonatal Suffolk sheep with the agent of classical scrapie results in PrPSc accumulation in sheep with the PRNP ARQ/ARQ but not the ARQ/ARR genotype
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Oral inoculation of neonatal Suffolk sheep with the agent of classical scrapie results in PrPSc accumulation in sheep with the PRNP ARQ/ARQ but not the ARQ/ARR genotype

机译：用经典瘙痒病试剂对新生儿萨福克羊进行口服接种会导致PRNP ARQ / ARQ但不是ARQ / ARR基因型的绵羊体内积累PrPSc

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摘要

Scrapie is a transmissible spongiform encephalopathy that can be transmitted amongst susceptible sheep. The prion protein gene (PRNP) profoundly influences the susceptibility of sheep to the scrapie agent. This study reports the failure to detect PrPSc in nervous or lymphoid tissues of Suffolk sheep of the PRNP ARQ/ARR genotype after oral inoculation with a U.S. scrapie isolate. Lambs were inoculated within the first 24 h of birth with 1 ml of a 10% (wt./vol.) brain homogenate derived from a clinically affected ARQ/ARQ sheep. The inoculated sheep were observed daily throughout the experiment for clinical signs suggestive of scrapie until they were necropsied at 86 months post inoculation. Tissues were collected for examination by immunohistochemistry and enzyme immunoassay, but all failed to demonstrate evidence of scrapie infection. Neonatal sheep of the ARQ/ARQ genotype receiving the same inoculum developed scrapie within 24 months. Lambs of the ARQ/ARR genotype that received the same inoculum by intracranial inoculation develop scrapie with a prolonged incubation period and with abnormal prion present within the central nervous system, but not peripheral lymphoid tissues. Results of this study suggest that ARQ/ARR sheep are resistant to oral infection with the scrapie isolate used even during the neonatal period. Published by Elsevier Ltd.
机译：瘙痒病是一种可传播的海绵状脑病，可在易感羊之间传播。 ion病毒蛋白基因（PRNP）深刻影响绵羊对瘙痒病的敏感性。这项研究报告了在口服美国羊scrap隔离株后，在PRNP ARQ / ARR基因型的萨福克绵羊的神经或淋巴组织中未能检测到PrPSc。在出生后的头24小时内，用从临床上受感染的ARQ / ARQ绵羊中提取的1 ml 10％（重量/体积）脑匀浆接种小羊。在整个实验过程中每天都观察到被接种的绵羊的临床症状，表明它们有瘙痒病，直到在接种后86个月进行尸检。收集组织进行免疫组织化学和酶免疫法检查，但均未显示出瘙痒病感染的证据。接受相同接种量的ARQ / ARQ基因型新生绵羊在24个月内出现瘙痒病。通过颅内接种接受相同接种物的ARQ / ARR基因型羔羊会出现瘙痒病，其潜伏期延长且中枢神经系统内存在异常pr病毒，但外周淋巴组织却没有。这项研究的结果表明，即使在新生儿期，ARQ / ARR绵羊也可以使用瘙痒病隔离株抵抗口腔感染。由Elsevier Ltd.发布

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