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首页> 外文期刊>Reproduction, fertility, and development >Evaluation of maize grain and polyunsaturated fatty acid (PUFA) as energy sources for breeding rams based on hormonal, sperm functional parameters and fertility.
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Evaluation of maize grain and polyunsaturated fatty acid (PUFA) as energy sources for breeding rams based on hormonal, sperm functional parameters and fertility.

机译：基于荷尔蒙，精子功能参数和繁殖力，评估玉米籽粒和多不饱和脂肪酸（PUFA）作为种公羊的能源。

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摘要

The objective of the present study was to elucidate the effect of different sources of dietary energy (maize vs polyunsaturated fatty acid (PUFA) on semen functional parameters and fertility of adult rams. Eighteen adult rams were divided into two groups (maize and PUFA, n=9). The main energy source for the rams in the maize group was coarsely ground maize grain, whereas in the PUFA group it was sunflower oil (rich in 18:2 linoleic acid, an omega-6 acid). The ration was fed for a minimum period of 60 days and thereafter semen was collected for evaluation. The proportion of progressive forward motility was significantly (P<0.05) higher in the PUFA group compared with the maize group. Sperm lipid peroxidation as measured by malondialdehyde formation (μM per 1×10(9) spermatozoa) was significantly (P<0.05) higher in the PUFA group compared with the maize group. When the semen was diluted with Tris-egg yolk-citrate buffer and incubated for 24h at 4°C, the proportions of plasmalemma integrity, the sperm subpopulation positive for functional membrane and acrosomal integrities, and mitochondrial membrane potential were significantly (P<0.05) higher in PUFA-fed than in maize-fed animals. The different sources of energy did not influence the serum and seminal plasma IGF-I levels. The cleavage rate (percentage) did not differ significantly between PUFA- (45.4±4.91) and maize- (44.63±6.8) fed animals. In conclusion, PUFA feeding influenced sperm quality by altering or stabilising membrane integrity. The present study indicates that PUFA may improve semen quality but did not improve in vitro fertilisation.
机译：本研究的目的是阐明不同饮食能量来源（玉米vs多不饱和脂肪酸（PUFA）对成年公羊精液功能参数和生育力的影响。18只成年公羊分为两组（玉米和PUFA，n = 9）。玉米组中公羊的主要能源是粗磨玉米粒，而PUFA组中则是葵花籽油（富含18：2的亚油酸，ω-6酸）。至少60天，然后收集精液进行评估。PUFA组的前进性向前运动比例明显高于玉米组（P <0.05）。通过丙二醛形成测量的精子脂质过氧化（μM PUFA组的1×10（9）精子比玉米组高（P <0.05），精子用Tris-蛋黄柠檬酸缓冲液稀释并在4°C温育24小时，质膜完整性y，在PUFA喂养的动物中，功能膜和顶体完整性阳性的精子亚群以及线粒体膜电位均显着（P <0.05）高。不同的能量来源不影响血清和精浆的IGF-I水平。 PUFA-（45.4±4.91）和玉米（44.63±6.8）喂养的动物之间的卵裂率（百分比）没有显着差异。总之，PUFA喂养通过改变或稳定膜的完整性来影响精子质量。本研究表明PUFA可以改善精液质量，但不能改善体外受精。

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