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Molecular and virological studies on contagious pustular dermatitis isolates from Egyptian sheep and goats.

机译:埃及绵羊和山羊的传染性脓疱性皮炎分离株的分子和病​​毒学研究。

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摘要

Orf virus was clinically diagnosed from different field cases of sheep and goat in Hawamdia, Giza, Egypt during the summer 2006. Skin scabs were collected and used for virus isolation, electron microscopy, PCR and sequencing for confirmation, and differential diagnosis. The aetiological virus was fruitfully isolated on the chorio-allantoic membrane of SPF embryonated chicken eggs indicated by expressing the characteristic pock lesions of Poxviridae family. Electron microscopy examination exposed negatively stained oval-shape virus particles trait for members of the genus Parapoxvirus. A 392 bp fragment of the late transcription factor (VLTF-1) gene of orf virus was amplified by PCR from the DNA extracted from the isolates. Phylogenetic analysis revealed 99% identity with other orf virus strains reported worldwide. Selection and processing of clinical specimens and PCR assay applied in this endeavor, presented a reliable laboratory diagnostic tool for orf infections and first molecular characterization of Egyptian orf isolates.
机译:在2006年夏季,从埃及吉萨的哈瓦姆迪亚的绵羊和山羊的不同田间病例中临床诊断出Orf病毒。收集了皮肤sc疮,并将其用于病毒分离,电子显微镜,PCR和测序以进行确认和鉴别诊断。通过表达Poxviridae家族的特征性鸡痘病变表明,在SPF胚胎鸡蛋的绒毛膜上成功分离出了病原病毒。电子显微镜检查显示副痘病毒属成员的负染椭圆形病毒颗粒特征。通过PCR从分离株提取的DNA中扩增出orf病毒的晚期转录因子(VLTF-1)基因的392bp片段。系统发育分析表明,与全世界报道的其他orf病毒株具有99%的同一性。临床标本的选择和处理以及在此过程中应用的PCR测定法,为orf感染和埃及orf分离株的首次分子表征提供了可靠的实验室诊断工具。

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