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首页> 外文期刊>Cell communication & adhesion >Adhesion of Monocytes to Medical Steel as Used for Vascular Stents is Mediated by the Integrin Receptor Mac-1 (CD11b/CD18; α_Mβ_2) and Can be Inhibited by Semiconductor Coating
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Adhesion of Monocytes to Medical Steel as Used for Vascular Stents is Mediated by the Integrin Receptor Mac-1 (CD11b/CD18; α_Mβ_2) and Can be Inhibited by Semiconductor Coating

机译：整合素受体Mac-1（CD11b / CD18;α_Mβ_2）介导单核细胞与用于血管支架的医用钢之间的粘附，并且可以被半导体涂层抑制

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Implantation of stents into stenosed arteries helps to restore normal blood flow in ischemic organs. However, limited biocompatibility of the applied medical steel can cause acute thrombosis and long-term restenosis. Adhesion of monocytes to stent metal may participate in those acute and long-term complications of stent placement. Based on described prominent electrochemical properties of the interaction between the monocyte integrin receptor Mac-1 and its various ligands, we hypothesized, that this receptor is a central mediator of monocyte adhesion to stent metal and that semiconductor coating of medical steel reduces monocyte adhesion. Adhesion of monocytes on L-316 stainless steel was directly evaluated by light microscopy. Mac-1 could be identified as mediator of monocyte adhesion, since cell adhesion could be blocked by anti-Mac-1-antibodies, including the cross-reacting anti-GPIIb/IIa antibody fragment abciximab. To further prove the central role of Mac-1, two CHO cell lines were generated expressing recombinant Mac-1 either as wild type, resulting in a low affinity receptor, or mutant with a GFFKR deletion of the α_M subunit, resulting in a high affinity receptor. Indeed, adhesion was specific for Mac-1 and dependent on the affinity state of this integrin. Finally, we could demonstrate that Mac-1 -mediated adhesion of monocytes to stents can be significantly inhibited by silicon carbide coating of the stent metal. In conclusion, the integrin Mac-1 and its affinity state could identified as major mediators of monocyte adhesion on medical steel. As therapeutic strategies, the blockade of Mac-1 by antibodies or silicon carbide coating of steel inhibits monocyte adhesion on stents.
机译：将支架植入狭窄的动脉有助于恢复缺血器官的正常血流。但是，所用医用钢的生物相容性有限会导致急性血栓形成和长期再狭窄。单核细胞与支架金属的粘附可能参与支架放置的那些急性和长期并发症。基于单核细胞整合素受体Mac-1及其各种配体之间相互作用的显着电化学特性，我们假设该受体是单核细胞粘附于支架金属的主要介质，医用钢的半导体涂层可降低单核细胞粘附。通过光学显微镜直接评估单核细胞在L-316不锈钢上的附着力。 Mac-1可被鉴定为单核细胞粘附的介体，因为细胞粘附可能被抗Mac-1-抗体（包括交叉反应的抗GPIIb / IIa抗体片段abciximab）阻断。为了进一步证明Mac-1的核心作用，生成了两种表达重组Mac-1的CHO细胞系，它们要么是野生型，导致亲和力低，要么是突变体，其中α_M亚基缺失了GFFKR，导致了亲和力高。受体。确实，粘附对于Mac-1是特定的，并且取决于该整联蛋白的亲和力状态。最后，我们可以证明支架金属的碳化硅涂层可以显着抑制Mac-1介导的单核细胞与支架的粘附。总之，整联蛋白Mac-1及其亲和力状态可以确定为医用钢单核细胞粘附的主要介质。作为治疗策略，通过抗体或钢的碳化硅涂层来阻止Mac-1可以抑制单核细胞粘附在支架上。

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