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Retinaldehyde, a Potent Inhibitor of Gap Junctional Intercellular Communication

机译：视黄醛，间隙连接细胞间通讯的有效抑制剂。

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摘要

Retinaldehyde and retinoic acid are derivatives of vitamin A, and retinaldehyde is the precursor for the synthesis of retinoic acid, a well-known inhibitor of gap junctional intercellular communication. In this investigation, we asked the question if retinaldehyde has similar effects on gap junctions. Gap junctional intercellular communication was measured by scrape-loading and preloading dye-transfer methods, and studies were carried out mainly on cultured liver epithelial cells. Retinaldehyde was found to be a more potent inhibitor (dye transfer reduced by 50% at 2.8 μM) than retinoic acid (dye transfer reduced by 50% at 30 μM) and glycyrrhetinic acid (dye transfer reduced by 50% at 65 μM). Both the 11-cis and all-trans forms of retinaldehyde were equally effective. Retinaldehyde inhibited dye transfer of both anionic Lucifer yellow and cationic Neurobiotin. Inhibition by retinaldehyde developed in less than two minutes at 50 μM, but unlike the reported case with retinoic acid, recovery was slower, though full. In addition to liver epithelial cells, retinaldehyde inhibited gap junctional communication in lens epithelial cells, retinal pigment epithelial cells and retinal ganglion cells.
机译：视黄醛和视黄酸是维生素A的衍生物，视黄醛是合成视黄酸的前体，视黄酸是众所周知的间隙连接细胞间通讯抑制剂。在这项调查中，我们问了视黄醛是否对间隙连接有类似的影响。间隙连接细胞间通讯通过刮擦加载和预加载染料转移方法进行了测量，并且研究主要在培养的肝上皮细胞上进行。发现视黄醛是更有效的抑制剂（在2.8μM时染料转移降低50％）比在视黄酸（在30μM时染料转移降低50％）和甘草次酸（在65μM时染料转移降低50％）更有效。视黄醛的11-顺式和全反式均有效。视黄醛抑制阴离子荧光素黄和阳离子神经生物素的染料转移。视黄醛的抑制作用在不到50分钟的两分钟内就产生了，但与报道的视黄酸不同，其恢复较慢，尽管已完成。除了肝上皮细胞，视黄醛还抑制晶状体上皮细胞，视网膜色素上皮细胞和视网膜神经节细胞中的间隙连接通讯。

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