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The Detection of Hamster Connexins: A Comparison of Expression Profiles with Wild-Type Mouse and the Cancer-Prone Min Mouse

机译:仓鼠连接蛋白的检测:与野生型小鼠和癌症无性系min小鼠表达谱的比较。

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The open reading frames of 17 connexins from Syrian hamster (using tissues) and 16 connexins from the Chinese hamster cell line V79, were fully (Cx30, Cx31, Cx37, Cx43 and Cx45) or partially sequenced. We have also detected, and partially sequenced, seven rat connexins that previously were unavailable. The expression of connexin genes was examined in some hamster organs and cultured hamster cells, and compared with wild-type mouse and the cancer-prone Min mouse. Although the expression patterns were similar for most organs and connexins in hamster and mouse, there were also some prominent differences (Cx29 and 30.3 in testis; Cx31.1 and 32 in eye; Cx46 in brain, kidney and testis; Cx47 in kidney). This suggests that some connexins have species-specific expression profiles. In contrast, there were minimal differences in expression profiles between wild type and Min mice. Species-specific expression profiles should be considered in attempts to make animal models of human connexin-associated diseases.
机译:来自叙利亚仓鼠的17种连接蛋白(使用组织)和来自中国仓鼠细胞系V79的16种连接蛋白的开放阅读框完全(Cx30,Cx31,Cx37,Cx43和Cx45)或部分测序。我们还检测到了先前无法获得的7种大鼠连接蛋白并进行了部分测序。检查了某些仓鼠器官和培养的仓鼠细胞中连接蛋白基因的表达,并与野生型小鼠和易患癌的Min小鼠进行了比较。尽管仓鼠和小鼠中大多数器官和连接蛋白的表达模式相似,但也存在一些显着差异(睾丸中Cx29和30.3;眼睛中Cx31.1和32;大脑,肾脏和睾丸中Cx46;肾脏中Cx47)。这表明某些连接蛋白具有物种特异性的表达谱。相反,野生型和Min小鼠之间的表达谱差异很小。在建立人连接蛋白相关疾病的动物模型时,应考虑物种特异性的表达谱。

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