...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell cycle >Nuclear actin in transcriptional reprogramming by oocytes: are actin nucleators key players?
【24h】

Nuclear actin in transcriptional reprogramming by oocytes: are actin nucleators key players?

机译：卵母细胞转录重编程中的核肌动蛋白：肌动蛋白成核剂是关键角色吗？

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Differentiated cells can be experimentally reprogrammed to an embryonic state by different approaches.Nuclear repro-gramming entails reactivation of silenced embryonic genes, such as Oct4 (Pou5f1). The most efficient method for reprogram-ming is regarded as nuclear transfer to eggs/oocytes. However, oocyte factors and mechanisms participating in this efficient reprogramming have not been elucidated. We have recently identified actin in a nucleus (nuclear actin) as one such factor by using the unique nuclear transfer system into Xenopus oocytes.Actin is a major constituent of the cytoskeleton. Growing evidence suggests that actin plays an important role in many nuclear events, including transcription, RNA processing, gene movement and chromatin remodeling.In our recent study, we took advantage of the giant nucleus of the Xenopus oocyte, referred to as the germinal vesicle (GV), in order to analyze functions of nuclear actin in transcriptional reprogramming (Fig. 1). Hundreds of somatic nuclei can be injected into one GV, and almost all transplanted nuclei are efficiently reprogrammed to express many embryonic genes.
机译：可以通过不同的方法将分化的细胞实验性重编程为胚胎状态。核重编程需要重新激活沉默的胚胎基因，例如Oct4（Pou5f1）。重新编程最有效的方法被认为是核转移至卵/卵母细胞。但是，尚未阐明参与这种有效重编程的卵母细胞因子和机制。最近，我们通过使用独特的到爪蟾卵母细胞的核转移系统，将细胞核中的肌动蛋白（核肌动蛋白）鉴定为一种这样的因子。肌动蛋白是细胞骨架的主要组成部分。越来越多的证据表明肌动蛋白在许多核事件中起着重要作用，包括转录，RNA加工，基因移动和染色质重塑。在我们最近的研究中，我们利用了非洲爪蟾卵母细胞的巨大核，称为生发囊泡（ GV），以分析核肌动蛋白在转录重编程中的功能（图1）。可以将数百个体细胞核注射到一个GV中，几乎所有移植的细胞核都可以有效地重新编程以表达许多胚胎基因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号