...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell cycle >Requirement of aurora-A kinase in astral microtubule polymerization and spindle microtubule flux.
【24h】

Requirement of aurora-A kinase in astral microtubule polymerization and spindle microtubule flux.

机译：星形微管聚合和纺锤体微管通量中极光A激酶的需求。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mitotic Aurora-A kinase was found to be required for formation of bipolar spindle, ensuring accurate chromosome segregation in mitosis. Recently, Aurora-A was shown to promote Ran-GTP-induced spindle formation and astral microtubule development. Here, by selective immunodepletion, we showed that Aurora-A was required for centrosome- but not Ran-GTP-induced astral microtubule formation in Xenopus egg extracts. Aurora-A enhanced microtubule polymerization in both centrosome- and Ran-GTP-induced aster assemblies: shortening the timing of aster assembly and increasing the aster size. Indeed, adding of Aurora-A protein alone induced microtubule clustering, which was abrogated by Aurora kinase inhibitory small molecule ZM447439. In addition, we showed that Aurora-A was indispensable for Ran-GTP-induced bipolar spindle formation. Inhibition of Aurora-A activity by adding of kinase inactive dominant mutant led to spindle collapse and formation of monopolar spindle whereas minus-end motor protein dynein/dynactin inhibitor p50/dynamitin rescued the bipolar structure. Lastly, we revealed that Aurora-A was necessary for microtubule poleward flux and this requirement depended on kinase activity. Thus, we showed that Aurora-A promoted microtubule polymerization and maintained microtubule flux in ensuring proper bipolar spindle assembly.
机译：发现双极纺锤体形成需要有丝分裂的Aurora-A激酶，以确保有丝分裂中染色体的准确分离。最近，Aurora-A被证明可以促进Ran-GTP诱导的纺锤体形成和星状微管发育。在这里，通过选择性的免疫耗竭，我们表明在非洲爪蟾卵提取物中，Aurora-A是中心体而不是Ran-GTP诱导的星状微管形成所必需的。 Aurora-A在中心体和Ran-GTP诱导的aster装配中增强了微管聚合：缩短了aster装配的时间并增加了aster尺寸。确实，单独添加Aurora-A蛋白会诱导微管聚集，而Aurora激酶抑制性小分子ZM447439则消除了这种聚集。此外，我们表明Aurora-A对于Ran-GTP诱导的双极纺锤体形成是必不可少的。通过添加激酶失活的显性突变体抑制Aurora-A活性导致纺锤体崩溃和单极纺锤体的形成，而负端运动蛋白动力蛋白/动力蛋白抑制剂p50 / dynamitin挽救了双极结构。最后，我们揭示了Aurora-A是微管极向通量所必需的，并且该要求取决于激酶活性。因此，我们表明Aurora-A在确保正确的双极纺锤体组装中促进了微管聚合并保持了微管通量。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号