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首页> 外文期刊>Cellular Physiology and Biochemistry >Mammalian AT2 Receptors Expressed in Xenopus laevis Oocytes Couple to Endogenous Chloride Channels and Stimulate Germinal Vesicle Break Down
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Mammalian AT2 Receptors Expressed in Xenopus laevis Oocytes Couple to Endogenous Chloride Channels and Stimulate Germinal Vesicle Break Down

机译：在非洲爪蟾卵母细胞中表达的哺乳动物AT2受体与内源性氯离子通道结合并刺激生殖小泡的分解

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Background and aims: A comparative analysis was performed of the properties of cloned AT1 and AT2 receptors and their ability to induce ion currents and oocyte maturation. Methods: Frog oocytes were injected with cRNA that codes for rat AT1A and AT2 and bovine AT1 receptors and membrane currents were recorded by using the two-microelectrode voltage-clamp technique. Oocyte maturation was scored by observation of the germinal vesicle breakdown (GVBD). Results: Xenopus oocytes expressing AT1 or AT2 generated oscillatory chloride currents by coupling to the diacylglycerol/inositol-3-phosphate (DAG/IP3) cascade. Ang II activation of collagenase-defolliculated oocytes expressing rat AT1A yielded larger chloride currents (9.2 +/- 3.4 A) than those generated by oocytes expressing bovine AT1 (3.78 +/- 2.6 A). Oocytes expressing rat AT1A were recovered from desensitization after 0.5 h and completely after 10.5 h; whereas oocytes expressing bovine AT1 receptors were unable to do so. Expression of rat AT2 generated smaller chloride currents (32-210 nA). Expression of AT2 receptors triggered GVBD whereas AT1 did not. The proportion of oocytes developing GVBD was greater for folliculated oocytes than for oocytes that were defolliculated. Furthermore, oocytes injected with AT2 secreted into the media, a heat-resistant factor(s) that stimulated GVBD of oocytes. This media elicited inward and outward membrane currents when applied to non-injected oocytes. Conclusion: Activation of AT1 and AT2 receptors couple to similar metabolic cascades in Xenopus laevis oocytes. However, the pathway activated by AT2 diverges to induce oocyte maturation. Copyright
机译：背景与目的：对克隆的AT1和AT2受体的性质及其诱导离子流和卵母细胞成熟的能力进行了比较分析。方法：给青蛙卵母细胞注射编码大鼠AT1A和AT2的cRNA，并用双微电极电压钳技术记录牛AT1受体的膜电流。卵母细胞的成熟是通过观察生胚囊泡破裂（GVBD）来评分的。结果：表达AT1或AT2的非洲爪蟾卵母细胞通过与二酰基甘油/肌醇-3-磷酸酯（DAG / IP3）级联反应产生振荡的氯化​​物电流。表达大鼠AT1A的胶原酶脱卵母细胞的Ang II活化产生的氯化物电流（9.2 +/- 3.4 A）比表达牛AT1的卵母细胞（3.78 +/- 2.6 A）更大。在0.5h后脱敏，并在10.5h后完全恢复表达大鼠AT1A的卵母细胞。而表达牛AT1受体的卵母细胞则不能。大鼠AT2的表达产生较小的氯离子电流（32-210 nA）。 AT2受体的表达触发了GVBD，而AT1则没有。卵泡化卵母细胞的卵泡发育GVBD的比例要大于卵泡化卵母细胞的比例。此外，注射有AT2的卵母细胞分泌到培养基中，这是一种刺激卵母细胞GVBD的耐热因子。当将这种介质应用于未注射的卵母细胞时，会引起向内和向外的膜电流。结论：非洲爪蟾卵母细胞中AT1和AT2受体的激活与相似的代谢级联反应有关。但是，AT2激活的途径发散，诱导卵母细胞成熟。版权

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