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首页> 外文期刊>Cell cycle >Involvement of Polo-like kinase 1 in MEK1/2-regulated spindle formation during mouse oocyte meiosis.
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Involvement of Polo-like kinase 1 in MEK1/2-regulated spindle formation during mouse oocyte meiosis.

机译：Polo样激酶1参与MEK1 / 2调控小鼠卵母细胞减数分裂过程中纺锤体的形成。

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Our recent studies have shown that MEK1/2 is a critical regulator of microtubule organization and spindle formation during oocyte meiosis. In the present study, we found that Plk1 colocalized with p-MEK1/2 at various meiotic stages after GVBD when microtubule began to organize. Also, Plk1 was able to coimmunoprecipitate with p-MEK1/2 in metaphase I stage mouse oocyte extracts, further confirming their physical interaction. Taxol-treated oocytes exhibited a number of cytoplasmic asters, in which both Plk1 and p-MEK1/2 were present, indicating that they might be complexed to participate in the acentrosomal spindle formation at the MTOCs during oocyte meiosis. Depolymerization of microtubules by nocodazole resulted in the complete disassembly of spindles, but Plk1 remained associated with p-MEK1/2, accumulating in the vicinity of chromosomes. More importantly, when p-MEK1/2 activity was blocked by U0126, Plk1 lost its normal localization at the spindle poles, which might be one of the most vital factors causing the abnormal spindles in MEK1/2-inhibited oocytes. Taken together, these data indicate that Plk1 and MEK1/2 regulate the spindle formation in the same pathway and that Plk1 is involved in MEK1/2-regulated spindle assembly during mouse oocyte meiotic maturation.
机译：我们最近的研究表明，MEK1 / 2是卵母细胞减数分裂过程中微管组织和纺锤体形成的关键调节剂。在本研究中，我们发现当微管开始组织时，Plk1在GVBD后的各个减数分裂阶段与p-MEK1 / 2共定位。同样，Plk1能够在中期I期小鼠卵母细胞提取物中与p-MEK1 / 2共免疫沉淀，进一步证实了它们的物理相互作用。紫杉醇处理的卵母细胞表现出许多胞质紫苑，其中同时存在Plk1和p-MEK1 / 2，这表明它们可能复合参与卵母细胞减数分裂期间MTOC的无纺锤体形成。诺考达唑使微管解聚导致纺锤体完全分解，但Plk1仍与p-MEK1 / 2相关，并在染色体附近积累。更重要的是，当U0126阻断p-MEK1 / 2活性时，Plk1失去了正常的纺锤体定位，这可能是引起MEK1 / 2抑制的卵母细胞异常纺锤体的最重要因素之一。两者合计，这些数据表明Plk1和MEK1 / 2在同一途径中调节纺锤体形成，并且Plk1在小鼠卵母细胞减数分裂成熟期间参与MEK1 / 2调节的纺锤体组装。

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