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首页> 外文期刊>Cell cycle >Laterally attached kinetochores recruit the checkpoint protein Bub1, but satisfy the spindle checkpoint.
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Laterally attached kinetochores recruit the checkpoint protein Bub1, but satisfy the spindle checkpoint.

机译：横向附着的动植物招募检查点蛋白Bub1，但满足纺锤体检查点。

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Kinetochore attachment to the ends of dynamic microtubules is a conserved feature of mitotic spindle organization that is thought to be critical for proper chromosome segregation. Although kinetochores have been described to transition from lateral to end-on attachments, the phase of lateral attachment has been difficult to study in yeast due to its transient nature. We have previously described a kinetochore mutant, DAM1-765, which exhibits lateral attachments and misregulation of microtubule length. Here we show that the misregulation of microtubule length in DAM1-765 cells occurs despite localization of microtubule associated proteins Bik1, Stu2, Cin8, and Kip3 to microtubules. DAM1-765 kinetochores recruit the spindle checkpoint protein Bub1, however Bub1 localization to DAM1-765 kinetochores is not sufficient to cause a cell cycle arrest. Interestingly, the DAM1-765 mutation rescues the temperature sensitivity of a biorientation-deficient ipl1-321 mutant, and DAM1-765 chromosome loss rates are similar to wild-type cells. The spindle checkpoint in DAM1-765 cells responds properly to unattached kinetochores created by nocodazole treatment and loss of tension caused by a cohesin mutant. Progression of DAM1-765 cells through mitosis therefore suggests that satisfaction of the checkpoint depends more highly on biorientation of sister kinetochores than on achievement of a specific interaction between kinetochores and microtubule plus ends.
机译：线粒体附着在动态微管的末端是有丝分裂纺锤体组织的保守特征，据认为对适当的染色体分离至关重要。尽管已经描述了动植物从侧向附着过渡到末端附着，但是由于其瞬时性质，在酵母中难以研究侧向附着的阶段。我们先前已经描述了一个线粒体突变体DAM1-765，它表现出侧向附着和微管长度的失调。在这里我们显示，尽管将微管相关蛋白Bik1，Stu2，Cin8和Kip3定位到微管，但DAM1-765细胞中微管长度的失调仍然发生。 DAM1-765动植物招募纺锤体检查点蛋白Bub1，但是Bub1定位于DAM1-765动植物却不足以引起细胞周期停滞。有趣的是，DAM1-765突变挽救了缺乏生物取向的ipl1-321突变体的温度敏感性，并且DAM1-765染色体的丢失率与野生型细胞相似。 DAM1-765细胞中的纺锤体检查点可对诺考达唑处理产生的未连接动植物和黏附素突变体引起的张力丧失做出适当反应。因此，DAM1-765细胞通过有丝分裂的进程表明，检查点的满意度在很大程度上取决于姐妹动植物的生物取向，而不是动植物与微管正末端之间特定相互作用的实现。

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