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首页> 外文期刊>Cell cycle >Spo5 phosphorylation is essential for its own timely degradation and for successful meiosis in Schizosaccharomyces pombe.
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Spo5 phosphorylation is essential for its own timely degradation and for successful meiosis in Schizosaccharomyces pombe.

机译：Spo5磷酸化对于其自身的及时降解和粟酒裂殖酵母的成功减数分裂至关重要。

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摘要

Protein phosphorylation is pivotal for meiotic progression, but little is known about its regulatory mechanisms. We show that before meiosis I, the meiosis-specific Schizosaccharomyces pombe protein Spo5 is phosphorylated in vivo on T29, T55, S59 and/or T63. In a mutant strain expressing Spo5 fused to green fluorescent protein with alanine substitutions of these amino acid sites (GFP; Spo5-4A-GFP), the timely degradation of Spo5 at meiosis II was not observed. Additionally, Spo5-4A-GFP signals were retained after metaphase II and were localized to the nucleus. This was accompanied by the nuclear mislocalization of Psy1, a marker of the forespore membrane (FSM), and the generation of empty cells, in which cytoplasm had leaked from the ruptured membrane, as well as by the appearance of asci harboring deformed spores. Indeed, thin-section electron microscopy (TEM) revealed fragile-looking spo5-4A-GFP ascospores with ruffled spore walls. In contrast, a mutant strain expressing a constitutively-phosphorylated form of Spo5 (Spo5-4D-GFP) was phenotypically indistinguishable from a strain expressing wild-type (WT) protein (Spo5-WT-GFP). Taken together, these results indicate that Spo5 phosphorylation ensures the timely degradation of Spo5 during meiosis and the proper localization of Psy1, leading to the production of viable spores with robust FSMs and strong walls.
机译：蛋白质磷酸化是减数分裂进程的关键，但对其调节机制知之甚少。我们显示，在减数分裂I之前，减数分裂特有的裂殖酵母菌pombe蛋白Spo5在体内在T29，T55，S59和/或T63上被磷酸化。在表达与绿色荧光蛋白融合的Spo5的突变株中，这些氨基酸位点被丙氨酸取代（GFP; Spo5-4A-GFP），未观察到减数分裂II时Spo5的及时降解。此外，Spo5-4A-GFP信号在中期II后得以保留，并定位于细胞核。这伴随着Psy1的核错位，Psy1是前孢子膜（FSM）的标记，空细胞的产生，其中细胞质已经从破裂的膜中泄漏出来，并且伴随着asci带有变形孢子的出现。确实，薄层电子显微镜（TEM）揭示了带有皱纹孢子壁的脆弱的spo5-4A-GFP子囊孢子。相比之下，表达Spo5组成型磷酸化形式（Spo5-4D-GFP）的突变菌株与表达野生型（WT）蛋白（Spo5-WT-GFP）的菌株在表型上没有区别。两者合计，这些结果表明，Spo5磷酸化可确保减数分裂期间Spo5的及时降解和Psy1的适当定位，从而导致产生具有坚固FSM和坚固壁的活孢子。

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